Publications (2) Datasheet COA
Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K12 antibody (A14227) at 1:500 dilution.HeLa cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human appendix using Acetyl-Histone H4-K12 antibody (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat ovary using Acetyl-Histone H4-K12 antibody (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using Acetyl-Histone H4-K12 antibody (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Acetyl-Histone H4-K12 antibody (A14227) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K12 antibody (A14227) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
| Product name | Acetyl-Histone H4-K12 Rabbit pAb |
|---|---|
| Catalog No. | A14227 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic acetylated peptide around K12 of human Histone H4 (NP_001029249.1). |
|---|---|
| Sequence | GLGKG |
| Gene ID | 8359 |
| Swiss prot | P62805 |
| Synonyms | H4/I; H4C1; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FI; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; HIST1H4B; Acetyl-Histone H4-K12 |
| Calculated MW | 11kDa |
| Observed MW | 11kDa |
| Reactivity | Human, Mouse, Rat, Other (Wide Range) |
|---|---|
| Tested applications | WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | C6, HeLa |
| Cellular location | Chromosome, Nucleus |
| Customer validation | WB (Rattus norvegicus, Saccharomyces cerevisiae) |
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Based on existing publications, the following genes are closely related to research on H4C2. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to H4C2. (Distance between topics and target gene indicate popularity.) H4C2
* Data provided by citexs.com, for reference only.
Publishing research using A14227? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
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