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Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Publications (23) Datasheet

ABclonal:Acetyl-Histone H3-K9 Rabbit pAb
ABclonal:Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (A7255) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours (left). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours (left). Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K9 antibody (A7255) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product nameAcetyl-Histone H3-K9 Rabbit pAb
Catalog No.A7255
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic peptide of human Acetyl-Histone H3-K9
SequenceEmail for sequence
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW17KDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
ChIP-seq 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, NIH/3T3, C6
Cellular locationChromosome, Nucleus
Customer validation

WB(Rattus norvegicus, Homo sapiens, Bombyx mori Linnaeus)

IHC(Rattus norvegicus)

ChIP(Mus musculus, Rattus norvegicus, Homo sapiens, Arabidopsis thaliana)

IP(Mus musculus, Rattus norvegicus)

IP(Rattus norvegicus)

ChIP-qPCR(Rattus norvegicus)

ABclonal:Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255) }

Western blot - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (A7255) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255) }

Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255) }

Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255) }

Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255) }

Immunohistochemistry - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255) }

Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours (left). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255) }

Immunofluorescence - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours (left). Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K9 Rabbit pAb (A7255) }

Chromatin Immunoprecipitation - Acetyl-Histone H3-K9 Rabbit pAb (A7255)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K9 antibody (A7255) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

* For research use only. Not for therapeutic or diagnostic purposes.

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