Reviews (2) Publications (7) Datasheet COA
Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Western blot analysis of HeLa, using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at 1:10000 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of NIH/3T3, using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at 1:10000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of C6, using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at 1:10000 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of C6 treated by TSA jia C6 cells using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 treated by TSA jia C6 cells using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 treated by TSA jia NIH-3T3 cells using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS treated by TSA jia U-2 OS cells using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of Hale cells, using Acetyl-Histone H3-K56 antibody (A7256) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Product name | Acetyl-Histone H3-K56 Rabbit pAb |
---|---|
Catalog No. | A7256 |
Host species | Rabbit |
Purification method | Affinity purification |
Isotype | IgG |
Reactivity | Human, Mouse, Rat, Other (Wide Range) |
---|---|
Tested applications | WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition |
Recommended dilution |
|
Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
Application key | Western blotting Immunofluorescence |
Positive samples | HeLa, NIH/3T3, C6 |
Cellular location | Chromosome, Nucleus |
Customer validation | IF (Mouse kidney cell line, Mus musculus) WB (Mouse kidney cell line, Homo sapiens, Saccharomyces cerevisiae, Mus musculus, Eurosta solidaginis) ChIP (Homo sapiens) |
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Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4
* Data provided by citexs.com, for reference only.
Publishing research using A7256? Please let us know so that we can cite the reference in this datasheet.
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K4 Rabbit pAb
A2357
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K9 Rabbit pAb
A2360
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K14 Rabbit pAb
A7254
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-K36 Rabbit pAb
A2364
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-K27 Rabbit pAb
A2361
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K79 Rabbit pAb
A2369
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb
A2421
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Phospho-Histone H3-S10 Rabbit pAb
AP0840
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb
A3155
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K36 Rabbit mAb
A20379
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K4 Rabbit pAb
A16078
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Lactic acid-Histone H3-K18 Rabbit pAb
A18807
Reactivity:Human, Mouse, Rat, Other (Wide Range)
DiMethyl-Histone H3-K27 Rabbit pAb
A2362
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-R26 Rabbit pAb
A3163
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-R2 Rabbit pAb
A3154
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Asymmetric DiMethyl-Histone H3-R26 Rabbit pAb
A2375
Reactivity:Human, Mouse, Rat, Other (Wide Range)
citrulline-Histone H3-R2/R8/R17 Rabbit pAb
A18298
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Lactic acid-Histone H3-K14 Rabbit pAb
A18808
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Lactic acid-Histone H3-K27 Rabbit pAb
A18825
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-K36 Rabbit pAb
A20566
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K23 Rabbit mAb
A2770
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Phospho-Histone H3-T11 Rabbit pAb
AP0093
Reactivity:Human, Mouse, Rat, Other (Wide Range)
succinylation-Histone H3-K79 Rabbit pAb
A17903
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K56 Rabbit pAb
A7262
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K64 Rabbit pAb
A7259
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-K36 Rabbit mAb
A23944
Reactivity:Human, Mouse, Rat, Other (Wide Range)
DiMethyl-Histone H3-K14 Rabbit pAb
A5278
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K14 Rabbit pAb
A5279
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-K14 Rabbit pAb
A5277
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Asymmetric DiMethyl-Histone H3-R8 Rabbit pAb
A3157
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Symmetric DiMethyl-Histone H3-R26 Rabbit pAb
A3153
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-R17 Rabbit pAb
A3151
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Symmetric DiMethyl-Histone H3-R17 Rabbit pAb
A3152
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb
A20732
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K36 Rabbit pAb
A16077
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K4/K9/K14/K18/K23/K27 Rabbit pAb
A21295
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K27 Rabbit mAb
A22396
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Phospho-Histone H3-S28 Rabbit pAb
AP0839
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb
A21299
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Phospho-Histone H3-S10/T11 Rabbit pAb
AP0896
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Phospho-Histone H3-T32 Rabbit pAb
AP0897
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Phospho-Histone H3-T6 Rabbit pAb
AP0899
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Phospho-Histone H3-T45 Rabbit pAb
AP0898
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K9/K14/K18/K23/K27 Rabbit pAb
A17917
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K23 Rabbit pAb
A18154
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-R2 Rabbit mAb
A19645
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Phospho-Histone H3-S10 Rabbit mAb
AP0002
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K27 Rabbit mAb
A22398
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K27 Rabbit pAb
A20184
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Symmetric DiMethyl-Histone H3-R8 Rabbit pAb
A20820
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-K79 Rabbit pAb
A20821
Reactivity:Human, Mouse, Rat, Other (Wide Range)
DiMethyl-Histone H3-K79 Rabbit pAb
A20822
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Symmetric DiMethyl-Histone H3-R8 Rabbit mAb
A21207
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Lactic acid-Histone H3-K18 Rabbit mAb
A21214
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-K79 Rabbit mAb
A21223
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K27 Rabbit mAb
A22006
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-K4 Rabbit mAb
A22078
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-K9 Rabbit mAb
A22079
Reactivity:Human, Mouse, Rat, Other (Wide Range)
DiMethyl-Histone H3-K36 Rabbit mAb
A22087
Reactivity:Human, Mouse, Rat, Other (Wide Range)
DiMethyl-Histone H3-K79 Rabbit mAb
A22142
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Asymmetric DiMethyl-Histone H3-R8 Rabbit mAb
A22144
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K56 Rabbit mAb
A22145
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-K27 Rabbit mAb
A22170
Reactivity:Human, Mouse, Rat, Other (Wide Range)
DiMethyl-Histone H3-K36 Rabbit mAb
A22221
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K4 Rabbit mAb
A22224
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K4 Rabbit mAb
A22225
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K4 Rabbit mAb
A22226
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Symmetric DiMethyl-Histone H3-R17 Rabbit mAb
A22291
Reactivity:Human, Mouse, Rat, Other (Wide Range)
TriMethyl-Histone H3-K9 Rabbit mAb
A22297
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K18 Rabbit mAb
A22566
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K9 Rabbit mAb
A22567
Reactivity:Human, Mouse, Rat, Other (Wide Range)
MonoMethyl-Histone H3-K36 Rabbit mAb
A22863
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Acetyl-Histone H3-K4 Rabbit mAb
A24341
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Histone H3 Rabbit mAb,sodium and BSA azide free
A25020FB
Reactivity:Human, Other (Wide Range)
* For research use only. Not for therapeutic or diagnostic purposes.