Reviews (2) Publications (7) Datasheet COA
Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Reactivity:Human, Mouse, Rat, Other (Wide Range)
Western blot analysis of HeLa, using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at 1:10000 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of NIH/3T3, using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at 1:10000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of C6, using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at 1:10000 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunofluorescence analysis of C6 treated by TSA jia C6 cells using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 treated by TSA jia C6 cells using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 treated by TSA jia NIH-3T3 cells using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS treated by TSA jia U-2 OS cells using Acetyl-Histone H3-K56 Rabbit pAb (A7256) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of Hale cells, using Acetyl-Histone H3-K56 antibody (A7256) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
| Product name | Acetyl-Histone H3-K56 Rabbit pAb |
|---|---|
| Catalog No. | A7256 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat, Other (Wide Range) |
|---|---|
| Tested applications | WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunofluorescence |
| Positive samples | HeLa, NIH/3T3, C6 |
| Cellular location | Chromosome, Nucleus |
| Customer validation | IF (Mouse kidney cell line, Mus musculus) WB (Mouse kidney cell line, Homo sapiens, Saccharomyces cerevisiae, Mus musculus, Eurosta solidaginis) ChIP (Homo sapiens) |
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Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4
* Data provided by citexs.com, for reference only.
Publishing research using A7256? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.
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