Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H3-K4 Rabbit pAb (A17019)

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Western blot - Acetyl-Histone H3-K4 Rabbit pAb (A17019)

Western blot analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 antibody (A17019) at 1:500 dilution.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 300s.

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Overview

Product name	Acetyl-Histone H3-K4 Rabbit pAb
Catalog No.	A17019
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic acetylated peptide around K4 of human Histone H3 (NP_003484.1).
Sequence	ARTKQ
Gene ID	8290 8350
Swiss prot	Q16695 P68431
Synonyms	H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Acetyl-Histone H3-K4
Calculated MW	16kDa
Observed MW	17kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key	Western blotting
Positive samples	HeLa
Cellular location	Chromosome, Nucleus
Customer validation	WB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Acetyl-Histone H3-K4 Rabbit pAb images

Western blot - Acetyl-Histone H3-K4 Rabbit pAb (A17019)

Product Protocols

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Related Targets

Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)

◀1/▶

Related Research Area

Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4

* Data provided by citexs.com, for reference only.