Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Review (1) Publications (48) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Acetyl-Histone H3-K27 Rabbit pAb
ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of NIH/3T3, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of C6, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2000 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded human colon using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded human spleen using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded rat lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Chromatin immunoprecipitation was performed with cross-linked chromatin from 293T, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG(AC005).The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram constructed the ratios of the ratio of the immunoprecipitated DNA versus the input.

Review
ABclonal: review for Acetyl-Histone H3-K27 Rabbit pAb(A7253)

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Overview

Product name Acetyl-Histone H3-K27 Rabbit pAb
Catalog No. A7253
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H3 (NP_003520.1).
Sequence KQLATKAARKSAPATGGVK
Gene ID 82908350
Swiss prot Q16695P68431
Synonyms H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Acetyl-Histone H3-K27
Calculated MW 15kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 1:50 - 1:200
  • ChIP 1:20 - 1:100
  • ChIP-seq 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples NIH/3T3, C6
Cellular location Chromosome, Nucleus
Customer validation

WB (Prawn gills, Rattus norvegicus, Danio rerio, Sus scrofa, Mus musculus, Homo sapiens, Monascus ruber, Oryza sativa Japonica Group, Eurosta solidaginis)

ChIP (Rattus norvegicus, Oryza sativa, Mus musculus, Gallus gallus, B. napus, Homo sapiens, Plasmodium)

IHC (Rattus norvegicus, Homo sapiens)

IP (Rattus norvegicus)

ChIP-Seq (Zea mays)

ChIP-qPCR (Rattus norvegicus)

IF (Homo sapiens, Mus musculus)

IP (Rattus norvegicus)

ChIP-seq (Homo sapiens)

Co-IP (Rattus norvegicus)

ChIP-PCR (Rattus norvegicus)

PCR (Homo sapiens)

WB (Homo sapiens, Plasmodium)

CHIP (Arabidopsis thaliana, Homo sapiens)

CoIP (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Acetyl-Histone H3-K27 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of NIH/3T3, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of C6, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2000 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded human colon using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded human spleen using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry analysis of paraffin-embedded rat lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Chromatin immunoprecipitation was performed with cross-linked chromatin from 293T, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG(AC005).The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram constructed the ratios of the ratio of the immunoprecipitated DNA versus the input.

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Related Targets

Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4

* Data provided by citexs.com, for reference only.

Publishing research using A7253? Please let us know so that we can cite the reference in this datasheet.

Antibodies (113)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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