Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Review (1)Publications (42) Datasheet SDS

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Acetyl-Histone H3-K27 Rabbit pAb
ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of NIH/3T3, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of C6, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2000 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded human spleen using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded mouse lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded rat lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for Acetyl-Histone H3-K27 Rabbit pAb(A7253)

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    Overview

    Product nameAcetyl-Histone H3-K27 Rabbit pAb
    Catalog No.A7253
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG

    Background

    Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

    Immunogen information

    ImmunogenA synthetic acetylated peptide around K27 of human Histone H3 (NP_003520.1).
    SequenceAARKS
    Gene ID8290
    Swiss protP68431
    SynonymsH3t; H3.4; H3/g; H3FT; H3C16; HIST3H3
    Calculated MW15kDa
    Observed MW17KDa

    Applications

    ReactivityHuman, Mouse, Rat, Other (Wide Range)
    Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition
    Recommended dilution
    • WB 1:1000 - 1:5000
    • IHC-P 1:50 - 1:200
    • IF/ICC 1:50 - 1:200
    • IP 1:50 - 1:200
    • ChIP 1:20 - 1:100
    • ChIP-seq 1:20 - 1:100
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
    Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
    Positive samplesNIH/3T3, C6
    Cellular locationChromosome, Nucleus
    Customer validation

    (Prawn gills)

    ChIP(Rattus norvegicus, Oryza sativa, Mus musculus, Gallus gallus, B. napus, Homo sapiens)

    IHC(Rattus norvegicus)

    IP(Rattus norvegicus)

    ChIP-Seq(Zea mays)

    WB(Rattus norvegicus, Danio rerio, Sus scrofa, Mus musculus, Homo sapiens, Monascus ruber, Oryza sativa Japonica Group)

    ChIP-qPCR(Rattus norvegicus)

    IF(Homo sapiens, Mus musculus)

    IP(Rattus norvegicus)

    ChIP-seq(Homo sapiens)

    CoIP(Rattus norvegicus)

    (Rattus norvegicus)

    Co-IP(Rattus norvegicus)

    ChIP-PCR(Rattus norvegicus)

    PCR(Homo sapiens)

    WB(Homo sapiens)

    CHIP(Arabidopsis thaliana, Homo sapiens)

    Research Area

    Acetyl-Histone H3-K27 Rabbit pAb images

    ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Western blot analysis of NIH/3T3, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Western blot analysis of C6, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2000 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunohistochemistry of paraffin-embedded human spleen using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunohistochemistry of paraffin-embedded mouse lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunohistochemistry of paraffin-embedded rat lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

    Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

    Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4

    * Data provided by citexs.com, for reference only.

    Publishing research using A7253? Please let us know so that we can cite the reference in this datasheet.

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