Review (1)Publications (39) Datasheet SDS
Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Other (Wide Range)
Western blot analysis of NIH/3T3, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2478 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of C6, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2478 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human spleen using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
| Product name | Acetyl-Histone H3-K27 Rabbit pAb |
|---|---|
| Catalog No. | A7253 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat, Other (Wide Range) |
|---|---|
| Tested applications | WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunoprecipitation |
| Positive samples | NIH/3T3, C6 |
| Cellular location | Chromosome, Nucleus |
| Customer validation | (Prawn gills) ChIP(Rattus norvegicus, Oryza sativa, Mus musculus, Gallus gallus, B. napus, Homo sapiens) IHC(Rattus norvegicus) IP(Rattus norvegicus) ChIP-Seq(Zea mays) WB(Rattus norvegicus, Danio rerio, Sus scrofa, Mus musculus, Homo sapiens, Monascus ruber) ChIP-qPCR(Rattus norvegicus) IF(Homo sapiens) IP(Rattus norvegicus) ChIP-seq(Homo sapiens) CoIP(Rattus norvegicus) (Rattus norvegicus) Co-IP(Rattus norvegicus) ChIP-PCR(Rattus norvegicus) PCR(Homo sapiens) WB(Homo sapiens) CHIP(Arabidopsis thaliana, Homo sapiens) |
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