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Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Review (1)Publications (29) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Acetyl-Histone H3-K27 Rabbit pAb
ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) at 1:1000 dilution.HeLa , NIH/3T3, and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded human breast using Acetyl-Histone H3-K27 antibody (A7253) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded rat heart using Acetyl-Histone H3-K27 antibody (A7253) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K27 antibody (A7253) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for Acetyl-Histone H3-K27 Rabbit pAb(A7253)

Overview

Product nameAcetyl-Histone H3-K27 Rabbit pAb
Catalog No.A7253
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic acetylated peptide around K27 of human Histone H3 (NP_003520.1).
SequenceAARKS
Gene ID8290
Swiss protP68431
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW17KDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF 1:50 - 1:200
  • IP 1:50 - 1:200
  • ChIP 1:20 - 1:100
  • ChIP-seq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, NIH/3T3, C6
Cellular locationChromosome, Nucleus
Customer validation

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ChIP(Rattus norvegicus, Oryza sativa, Mus musculus, Gallus gallus, B. napus, Homo sapiens)

IHC(Rattus norvegicus)

IP(Rattus norvegicus)

ChIP-Seq(Zea mays)

WB(Rattus norvegicus, Danio rerio, Sus scrofa)

ChIP-qPCR(Rattus norvegicus)

IF(Homo sapiens)

IP(Rattus norvegicus)

ChIP-seq(Homo sapiens)

CoIP(Rattus norvegicus)

(Rattus norvegicus)

Co-IP(Rattus norvegicus)

ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) at 1:1000 dilution.HeLa , NIH/3T3, and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded human breast using Acetyl-Histone H3-K27 antibody (A7253) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded rat heart using Acetyl-Histone H3-K27 antibody (A7253) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K27 antibody (A7253) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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