Review (1)Publications (23) Datasheet
Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Other (Wide Range)
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 antibody (A7253) at 1:1000 dilution.HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human breast using Acetyl-Histone H3-K27 antibody (A7253) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat heart using Acetyl-Histone H3-K27 antibody (A7253) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K27 antibody (A7253) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
| Product name | Acetyl-Histone H3-K27 Rabbit pAb |
|---|---|
| Catalog No. | A7253 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Reactivity | Human, Mouse, Rat, Other (Wide Range) |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 ChIP-seq 1:20 - 1:100 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
| Positive samples | BT-474, 293T |
| Cellular location | Chromosome, Nucleus |
| Customer validation | (Prawn gills) ChIP(Rattus norvegicus, Oryza sativa, Mus musculus, Gallus gallus) IHC(Rattus norvegicus) IP(Rattus norvegicus) ChIP-Seq(Zea mays) WB(Rattus norvegicus, Danio rerio) ChIP-qPCR(Rattus norvegicus) IF(Homo sapiens) IP(Rattus norvegicus) ChIP-seq(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
