Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Review (1)Publications (39) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Acetyl-Histone H3-K27 Rabbit pAb
ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of NIH/3T3, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2478 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of C6, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2478 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded human spleen using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded mouse lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded rat lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Review
ABclonal: review for Acetyl-Histone H3-K27 Rabbit pAb(A7253)

Overview

Product nameAcetyl-Histone H3-K27 Rabbit pAb
Catalog No.A7253
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

ImmunogenA synthetic acetylated peptide around K27 of human Histone H3 (NP_003520.1).
SequenceAARKS
Gene ID8290
Swiss protP68431
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW17KDa

Applications

ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 1:50 - 1:200
  • ChIP 1:20 - 1:100
  • ChIP-seq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesNIH/3T3, C6
Cellular locationChromosome, Nucleus
Customer validation

(Prawn gills)

ChIP(Rattus norvegicus, Oryza sativa, Mus musculus, Gallus gallus, B. napus, Homo sapiens)

IHC(Rattus norvegicus)

IP(Rattus norvegicus)

ChIP-Seq(Zea mays)

WB(Rattus norvegicus, Danio rerio, Sus scrofa, Mus musculus, Homo sapiens, Monascus ruber)

ChIP-qPCR(Rattus norvegicus)

IF(Homo sapiens)

IP(Rattus norvegicus)

ChIP-seq(Homo sapiens)

CoIP(Rattus norvegicus)

(Rattus norvegicus)

Co-IP(Rattus norvegicus)

ChIP-PCR(Rattus norvegicus)

PCR(Homo sapiens)

WB(Homo sapiens)

CHIP(Arabidopsis thaliana, Homo sapiens)

Research Area

Acetyl-Histone H3-K27 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of NIH/3T3, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2478 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Western blot analysis of C6, using Acetyl-HistoneH3-K27 antibody (A7253) at 1:2478 dilution.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded human spleen using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded mouse lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunohistochemistry - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunohistochemistry of paraffin-embedded rat lung using Acetyl-Histone H3-K27 Rabbit pAb (A7253) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of C6 cells treated by TSA (upper left) and untreated C6 cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of NIH-3T3 cells treated by TSA (upper left) and untreated NIH-3T3 cells(upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Immunofluorescence - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Immunofluorescence analysis of U-2 OS cells treated by TSA (upper left) and untreated U-2 OS cells (upper right) using Acetyl-Histone H3-K27 Rabbit pAb (red, A7253) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)}

Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A7253)

Chromatin immunoprecipitation analysis of extracts of HeLa; cells, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A7253) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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