Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H3-K27 Rabbit pAb (A20184)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A20184)

Western blot analysis of various lysates using Acetyl-Histone H3-K27 Rabbit pAb (A20184) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A20184)

Western blot analysis of various lysates using Acetyl-Histone H3-K27 Rabbit pAb (A20184) at 1:1000 dilution.HeLa cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Dot Blot - Acetyl-Histone H3-K27 Rabbit pAb (A20184)

Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K27 antibody (A20184) at 1:1000 dilution.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A20184)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A20184) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Overview

Product name Acetyl-Histone H3-K27 Rabbit pAb
Catalog No. A20184
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic acetylated peptide around K27 of human Histone H3 (NP_003520.1).
Sequence ARKSA
Gene ID 82908350
Swiss prot Q16695P68431
Synonyms H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; Acetyl-Histone H3-K27
Calculated MW 15kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • DB 1:500 - 1:1000
  • WB 1:500 - 1:1000
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, C6, NIH/3T3
Cellular location extracellular exosome, extracellular region, nucleoplasm, nucleus

Documents

Certificate of Compliance

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Lot number

Acetyl-Histone H3-K27 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A20184)}

Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A20184)

Western blot analysis of various lysates using Acetyl-Histone H3-K27 Rabbit pAb (A20184) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A20184)}

Western blot - Acetyl-Histone H3-K27 Rabbit pAb (A20184)

Western blot analysis of various lysates using Acetyl-Histone H3-K27 Rabbit pAb (A20184) at 1:1000 dilution.HeLa cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Dot Blot - Acetyl-Histone H3-K27 Rabbit pAb (A20184)}

Dot Blot - Acetyl-Histone H3-K27 Rabbit pAb (A20184)

Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K27 antibody (A20184) at 1:1000 dilution.
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A20184)}

Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 Rabbit pAb (A20184)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K27 Rabbit pAb antibody (A20184) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Related Targets

Based on existing publications, the following genes are closely related to research on H3C1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H3C1. (Distance between topics and target gene indicate popularity.) H3C1

* Data provided by citexs.com, for reference only.

Publishing research using A20184? Please let us know so that we can cite the reference in this datasheet.

Antibodies (114)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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