Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Review (1) Publications (25) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Western blot - Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K14 antibody (A7254) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H3-K14 Rabbit pAb (A7254) at dilution of 100 (40x lens). U-2 OS cells were treated by TSA (1 uM) at 37℃ for 18 hours.Blue: DAPI for nuclear staining.

Review
ABclonal: review for Acetyl-Histone H3-K14 Rabbit pAb(A7254)

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Overview

Product name Acetyl-Histone H3-K14 Rabbit pAb
Catalog No. A7254
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic acetylated peptide around K14 of human H3 (NP_003520.1).
Sequence GGKAP
Gene ID 82908350
Swiss prot Q16695P68431
Synonyms H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Acetyl-Histone H3-K14
Calculated MW 16kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, NIH/3T3, C6
Cellular location Chromosome, Nucleus
Customer validation

WB (HL-60, Rattus norvegicus, Mus musculus, Homo sapiens, Monascus ruber, A. fumigatus, Eurosta solidaginis)

ChIP (Rattus norvegicus, Homo sapiens, Mus musculus)

ChIP-qPCR (Rattus norvegicus, Homo sapiens)

IP (Rattus norvegicus)

FC (Saccharomyces cerevisiae)

Co-IP (Rattus norvegicus)

ChIP-PCR (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Acetyl-Histone H3-K14 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H3-K14 Rabbit pAb (A7254)}

Western blot - Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K14 antibody (A7254) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K14 Rabbit pAb (A7254)}

Immunofluorescence - Acetyl-Histone H3-K14 Rabbit pAb (A7254)

Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H3-K14 Rabbit pAb (A7254) at dilution of 100 (40x lens). U-2 OS cells were treated by TSA (1 uM) at 37℃ for 18 hours.Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4

* Data provided by citexs.com, for reference only.

Publishing research using A7254? Please let us know so that we can cite the reference in this datasheet.

Antibodies (113)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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