Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Western blot - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Western blot analysis of various lysates using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded rat spleen using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded human gastric cancer using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded mouse spleen using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Overview

Product name	Acetyl-Histone H2B-K5 Rabbit pAb
Catalog No.	A15621
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic acetylated peptide around K5 of human Histone H2B (NP_003519.1).
Sequence	PAKSA
Gene ID	3017 8349
Swiss prot	P62807 Q16778
Synonyms
Calculated MW
Observed MW	16kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	NIH/3T3, C6
Cellular location

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Acetyl-Histone H2B-K5 Rabbit pAb images

Western blot - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunohistochemistry - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunofluorescence - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Product Protocols

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Related Targets

Based on existing publications, the following genes are closely related to research on Histone H2B. (Numbers indicate number of published articles where both targets appear.)

◀1/▶

Related Research Area

Based on existing publications, the following research topics are related to Histone H2B. (Distance between topics and target gene indicate popularity.) Histone H2B

* Data provided by citexs.com, for reference only.