Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Western blot - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Western blot analysis of various lysates using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded rat spleen using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded human gastric cancer using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded mouse spleen using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Overview

Product name Acetyl-Histone H2B-K5 Rabbit pAb
Catalog No. A15621
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic acetylated peptide around K5 of human Histone H2B (NP_003519.1).
Sequence PAKSA
Gene ID 30178349
Swiss prot P62807Q16778
Synonyms
Calculated MW
Observed MW 16kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples NIH/3T3, C6
Cellular location

Documents

Certificate of Compliance

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Lot number

Acetyl-Histone H2B-K5 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)}

Western blot - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Western blot analysis of various lysates using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)}

Immunohistochemistry - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded rat spleen using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)}

Immunohistochemistry - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded human gastric cancer using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)}

Immunohistochemistry - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunohistochemistry analysis of Acetyl-Histone H2B-K5 in paraffin-embedded mouse spleen using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)}

Immunofluorescence - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)}

Immunofluorescence - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)}

Immunofluorescence - Acetyl-Histone H2B-K5 Rabbit pAb (A15621)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 Rabbit pAb (A15621) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on Histone H2B. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Histone H2B. (Distance between topics and target gene indicate popularity.) Histone H2B

* Data provided by citexs.com, for reference only.

Publishing research using A15621? Please let us know so that we can cite the reference in this datasheet.

Antibodies (11)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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