Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Western blot analysis of various lysates using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at 1:1000 dilution.HeLa cells and C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunohistochemistry analysis of Acetyl-Histone H2B-K12 in paraffin-embedded human colon using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunohistochemistry analysis of Acetyl-Histone H2B-K12 in paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunohistochemistry analysis of Acetyl-Histone H2B-K12 in paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours (top left). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours (top left). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours (top left). Blue: DAPI for nuclear staining.

ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H2B-K12 antibody (A15619) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

You may also interested in:

Overview

Product name Acetyl-Histone H2B-K12 Rabbit pAb
Catalog No. A15619
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.

Immunogen information

Immunogen A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1).
Sequence APKKG
Gene ID 30178349
Swiss prot P62807Q16778
Synonyms H2B; H2BE; H2BQ; GL105; H2B.1; H2BFQ; H2BGL105; H2B-GL105; HIST2H2BE; Acetyl-Histone H2B-K12
Calculated MW 14kDa
Observed MW 14kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, C2C12, C6
Cellular location Chromosome, Nucleus
Customer validation

WB (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Acetyl-Histone H2B-K12 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)}

Western blot - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Western blot analysis of various lysates using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at 1:1000 dilution.HeLa cells and C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)}

Immunohistochemistry - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunohistochemistry analysis of Acetyl-Histone H2B-K12 in paraffin-embedded human colon using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)}

Immunohistochemistry - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunohistochemistry analysis of Acetyl-Histone H2B-K12 in paraffin-embedded rat ovary using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)}

Immunohistochemistry - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunohistochemistry analysis of Acetyl-Histone H2B-K12 in paraffin-embedded mouse testis using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)}

Immunofluorescence - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours (top left). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)}

Immunofluorescence - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours (top left). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)}

Immunofluorescence - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K12 Rabbit pAb (A15619) at dilution of 1:100.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours (top left). Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)}

Chromatin Immunoprecipitation - Acetyl-Histone H2B-K12 Rabbit pAb (A15619)

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H2B-K12 antibody (A15619) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Inquire About This Product

Submit your question about A15619 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on H2BC21. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H2BC21. (Distance between topics and target gene indicate popularity.) H2BC21

* Data provided by citexs.com, for reference only.

Publishing research using A15619? Please let us know so that we can cite the reference in this datasheet.

Antibodies (11)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order