Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H2A-K9 Rabbit mAb (A11018)

Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - Acetyl-Histone H2A-K9 Rabbit mAb (A11018)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K9 antibody (A11018).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product nameAcetyl-Histone H2A-K9 Rabbit mAb
Catalog No.A11018
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Immunogen information

ImmunogenA specific peptide of human Acetyl-Histone H2A-K9
SequenceEmail for sequence
Gene ID30128335
Swiss protP04908
SynonymsH2A K9ac
Calculated MW14kDa
Observed MW14kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:500 - 1:1000
  • IF/ICC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular location

Acetyl-Histone H2A-K9 Rabbit mAb images

ABclonal:Western blot - Acetyl-Histone H2A-K9 Rabbit mAb (A11018)}

Western blot - Acetyl-Histone H2A-K9 Rabbit mAb (A11018)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K9 antibody (A11018).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on Histone H2A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Histone H2A. (Distance between topics and target gene indicate popularity.) Histone H2A

* Data provided by citexs.com, for reference only.

Publishing research using A11018? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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