Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Western blot - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K5 antibody (A15620) at 1:1000 dilution.C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Overview

Product name Acetyl-Histone H2A-K5 Rabbit pAb
Catalog No. A15620
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).
Sequence GRGKQ
Gene ID 30128329
Swiss prot P04908P0C0S8
Synonyms H2A/c; H2AFC; H2AC11; H2AC15; H2AC16; H2AC17; HIST1H2AI; Acetyl-Histone H2A-K5
Calculated MW 14kDa
Observed MW 14kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples C2C12, C6
Cellular location Chromosome, Nucleus

Documents

Certificate of Compliance

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Acetyl-Histone H2A-K5 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)}

Western blot - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K5 antibody (A15620) at 1:1000 dilution.C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)}

Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)}

Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on H2AC13. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H2AC13. (Distance between topics and target gene indicate popularity.) H2AC13

* Data provided by citexs.com, for reference only.

Publishing research using A15620? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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