Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Other (Wide Range)

ABclonal:Western blot - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K5 antibody (A15620) at 1:1000 dilution.C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

Overview

Product nameAcetyl-Histone H2A-K5 Rabbit pAb
Catalog No.A15620
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

ImmunogenA synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).
SequenceGRGKQ
Gene ID8329
Swiss protP0C0S8
SynonymsHIST1H2AI; H2A/c; H2AFC
Calculated MW14kDa
Observed MW14kDa

Applications

ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    
Positive samplesC2C12, C6
Cellular locationChromosome, Nucleus

Research Area

Acetyl-Histone H2A-K5 Rabbit pAb images

ABclonal:Western blot - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)}

Western blot - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K5 antibody (A15620) at 1:1000 dilution.C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)}

Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)}

Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on Histone H2A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Histone H2A. (Distance between topics and target gene indicate popularity.) Histone H2A

* Data provided by citexs.com, for reference only.

Publishing research using A15620? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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