Tag and Loading Control Antibodies
Nucleic Acid Modifying Enzymes
Ubiquitination related reagent
Cytokines & Cytokine Receptors
Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat, Other (Wide Range)
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K5 antibody (A15620) at 1:1000 dilution.C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
|Product name||Acetyl-Histone H2A-K5 Rabbit pAb|
|Purification method||Affinity purification|
|Reactivity||Human, Mouse, Rat, Other (Wide Range)|
|Tested applications||WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.|
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
|Application key||Western blotting|
|Positive samples||C2C12, C6|
|Cellular location||Chromosome, Nucleus|
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Based on existing publications, the following genes are closely related to research on Histone H2A. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to Histone H2A. (Distance between topics and target gene indicate popularity.) Histone H2A
* Data provided by citexs.com, for reference only.
Publishing research using A15620? Please let us know so that we can cite the reference in this datasheet.
* For research use only. Not for therapeutic or diagnostic purposes.