Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Tested applications:WB IHC IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIPReactivity:Human, Mouse, Rat, Other (Wide Range)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2A-K5 antibody (A15620) at 1:1000 dilution.C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.HeLa cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

Overview

Product name	Acetyl-Histone H2A-K5 Rabbit pAb
Catalog No.	A15620
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen	A synthetic acetylated peptide around K5 of human Histone H2A (NP_003508.1).
Sequence	GRGKQ
Gene ID	8329
Swiss prot	P0C0S8
Synonyms	HIST1H2AI; H2A/c; H2AFC
Calculated MW	14kDa
Observed MW	14kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range)
Tested applications	WB IHC IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application key	Western blotting
Positive samples	C2C12, C6
Cellular location	Chromosome, Nucleus

Research Area

Acetyl-Histone H2A-K5 Rabbit pAb images

Western blot - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 antibody (A15620) at dilution of 1:100.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.

Immunofluorescence - Acetyl-Histone H2A-K5 Rabbit pAb (A15620)

Product Protocols

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Related Targets

Based on existing publications, the following genes are closely related to research on Histone H2A. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to Histone H2A. (Distance between topics and target gene indicate popularity.) Histone H2A

* Data provided by citexs.com, for reference only.