Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunohistochemistry analysis of Acetyl-Histone H1.4-K26 in paraffin-embedded rat ovary using Acetyl-Histone H1.4-K26 Rabbit pAb (A17893) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunohistochemistry analysis of Acetyl-Histone H1.4-K26 in paraffin-embedded human tonsil using Acetyl-Histone H1.4-K26 Rabbit pAb (A17893) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunohistochemistry analysis of Acetyl-Histone H1.4-K26 in paraffin-embedded mouse brain using Acetyl-Histone H1.4-K26 Rabbit pAb (A17893) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H1.4-K26 (A17893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H1.4-K26 (A17893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H1.4-K26 (A17893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name Acetyl-Histone H1.4-K26 Rabbit pAb
Catalog No. A17893
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.

Immunogen information

Immunogen A synthetic acetylated peptide around K26 of human Histone H14 (NP_005312.1).
Sequence ARKSA
Gene ID 3008
Swiss prot P10412
Synonyms H1E; H1.4; H1F4; RMNS; H1s-4; HIST1H1E; dJ221C16.5; Acetyl-Histone H1.4-K26
Calculated MW 22kDa
Observed MW Refer to figures

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular location nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

Acetyl-Histone H1.4-K26 Rabbit pAb images

ABclonal:Immunohistochemistry - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)}

Immunohistochemistry - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunohistochemistry analysis of Acetyl-Histone H1.4-K26 in paraffin-embedded rat ovary using Acetyl-Histone H1.4-K26 Rabbit pAb (A17893) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)}

Immunohistochemistry - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunohistochemistry analysis of Acetyl-Histone H1.4-K26 in paraffin-embedded human tonsil using Acetyl-Histone H1.4-K26 Rabbit pAb (A17893) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)}

Immunohistochemistry - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunohistochemistry analysis of Acetyl-Histone H1.4-K26 in paraffin-embedded mouse brain using Acetyl-Histone H1.4-K26 Rabbit pAb (A17893) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)}

Immunofluorescence - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunofluorescence analysis of C6 cells using Acetyl-Histone H1.4-K26 (A17893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)}

Immunofluorescence - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H1.4-K26 (A17893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)}

Immunofluorescence - Acetyl-Histone H1.4-K26 Rabbit pAb (A17893)

Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H1.4-K26 (A17893) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on H1-4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H1-4. (Distance between topics and target gene indicate popularity.) H1-4

* Data provided by citexs.com, for reference only.

Publishing research using A17893? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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