Western blot analysis of extracts of HeLa cells, using Acetyl-FOXO1-K262/K265/K274 antibody (A17406) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.
|Product name||Acetyl-FOXO1-K262/K265/K274 Rabbit pAb|
|Purification method||Affinity purification|
|Tested applications||WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
|Application key||Western blotting|
|Cellular location||Cytoplasm, Nucleus|
WB (Gallus gallus, Mus musculus)
IF (Mus musculus)
WB (Mus musculus)
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Based on existing publications, the following genes are closely related to research on FOXO1. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to FOXO1. (Distance between topics and target gene indicate popularity.) FOXO1
* Data provided by citexs.com, for reference only.
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* For research use only. Not for therapeutic or diagnostic purposes.