Product Type > Antibodies > Primary Antibodies > Acetyl-specific Antibodies

Acetyl-FOXO1-K262/K265/K274 Rabbit pAb (A17406)

Publications (5) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Acetyl-FOXO1-K262/K265/K274 Rabbit pAb (A17406)

Western blot analysis of extracts of HeLa cells, using Acetyl-FOXO1-K262/K265/K274 antibody (A17406) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

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Overview

Product name Acetyl-FOXO1-K262/K265/K274 Rabbit pAb
Catalog No. A17406
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 221-320 of human FOXO1 (NP_002006.2).
Sequence SKFAKSRSRAAKKKAS
Gene ID 2308
Swiss prot Q12778
Synonyms FKH1; FKHR; FOXO1A; Acetyl-FOXO1-K262/K265/K274
Calculated MW 70kDa
Observed MW Refer to figures

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Gallus gallus, Mus musculus)

IF (Mus musculus)

WB (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Acetyl-FOXO1-K262/K265/K274 Rabbit pAb images

ABclonal:Western blot - Acetyl-FOXO1-K262/K265/K274 Rabbit pAb (A17406)}

Western blot - Acetyl-FOXO1-K262/K265/K274 Rabbit pAb (A17406)

Western blot analysis of extracts of HeLa cells, using Acetyl-FOXO1-K262/K265/K274 antibody (A17406) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on FOXO1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FOXO1. (Distance between topics and target gene indicate popularity.) FOXO1

* Data provided by citexs.com, for reference only.

Publishing research using A17406? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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