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Acetyl-Histone H4-K16 pAb (A5280)

Datasheet

ABclonal:Western blot - Acetyl-Histone H4-K16 pAb (A5280)

Overview

Product nameAcetyl-Histone H4-K16 pAb
Catalog No.A5280
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
ImmunogenA synthetic acetylated peptide around K16 of human Histone H4 (NP_001029249.1).
SequenceGGAKR
Gene ID8370
Swiss protP62805
SynonymsFO108; H4; H4/n; H4F2; H4FN; HIST2H4
Calculated MW11kDa
Observed MW13kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHela TSA, HeLa, C2C12 TSA, C2C12
Cellular locationChromosome, Nucleus
ABclonal:Western blot - Acetyl-Histone H4-K16 pAb (A5280) }

Western blot - Acetyl-Histone H4-K16 pAb (A5280)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K16 antibody (A5280) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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