Products > Catalog Antibodies > Acetylated Antibodies

Acetyl-Histone H3-K9 pAb (A7255)


ABclonal:Western blot - Acetyl-Histone H3-K9 pAb (A7255)
ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 pAb (A7255)
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K9 pAb (A7255)


Product nameAcetyl-Histone H3-K9 pAb
Catalog No.A7255
Host speciesRabbit
Purification methodAffinity purification
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic peptide of human Acetyl-Histone H3-K9
SequenceEmail for sequence
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t
Calculated MW15kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesBT-474, 293T, H3 protein
Cellular locationChromosome, Nucleus
ABclonal:Western blot - Acetyl-Histone H3-K9 pAb (A7255) }

Western blot - Acetyl-Histone H3-K9 pAb (A7255)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (A7255) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Acetyl-Histone H3-K9 pAb (A7255) }

Immunofluorescence - Acetyl-Histone H3-K9 pAb (A7255)

Immunofluorescence analysis of U2OS cells using Acetyl-Histone H3-K9 antibody (A7255) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K9 pAb (A7255) }

Chromatin Immunoprecipitation - Acetyl-Histone H3-K9 pAb (A7255)

Chromatin immunoprecipitation analysis extracts of 293 cell line, using H3K9ac antibody (A7255) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Submit an review for A7255

  1. Please choose your application, enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)

Reviews List

Inquire About This Product

Submit your question about A7255 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Select size and quantity
Contact us to order