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Acetyl-Histone H3-K27 pAb (A7253)

Review (1) Datasheet

ABclonal:Western blot - Acetyl-Histone H3-K27 pAb (A7253)
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 pAb (A7253)

Overview

Product nameAcetyl-Histone H3-K27 pAb
Catalog No.A7253
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic acetylated peptide corresponding to residues surrounding K27 of human Histone H3
SequenceEmail for sequence
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t
Calculated MW15kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
CHIPseq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesBT-474, 293T, H3 protein
Cellular locationChromosome, Nucleus
Customer validation

WB(Prawn gills)

ABclonal:Western blot - Acetyl-Histone H3-K27 pAb (A7253) }

Western blot - Acetyl-Histone H3-K27 pAb (A7253)

Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K27 antibody (A7253) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 pAb (A7253) }

Chromatin Immunoprecipitation - Acetyl-Histone H3-K27 pAb (A7253)

Chromatin immunoprecipitation analysis extracts of HeLa cells, using Acetyl-Histone H3-K27 antibody (A7253) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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