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ATRIP Polyclonal Antibody (A7139)

Datasheet

ABclonal:Western blot - ATRIP antibody (A7139)
ABclonal:Immunoprecipitation - 1ug ATRIP antibody (A7139)

Overview

Product nameATRIP Polyclonal Antibody
Catalog No.A7139
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ATRIP (NP_569055.1).
SequenceMAGTSAPGSKRRSEPPAPRPGPPPGTGHPPSKRARGFSAAAAPDPDDPFGAHGDFTADDLEELDTLASQALSQCPAAARDVSSDHKVHRLLDGMSKNPSGKNRETVPIKDNFELEVLQAQYKELKEKMKVMEEEVLIKNGEIKILRDSLHQTESVLEEQRRSHFLLEQEKTQALSDKEKEFSKKLQSLQSELQFKDAEMNELRTKLQTSERANKLAAPSVSHVSPRKNPSVVIKPEACSPQFGKTSFPTKESFSANMSLP
Gene ID84126
Swiss protQ8WXE1
Synonyms
Calculated MW72kDa/76kDa/83kDa/85kDa
Observed MW86kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesHepG2, Jurkat, HeLa, Mouse brain, Mouse heart
Cellular locationNucleus
ABclonal:Western blot - ATRIP antibody (A7139)}

Western blot - ATRIP antibody (A7139)

Western blot analysis of extracts of various cell lines, using ATRIP antibody (A7139) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 1s.
ABclonal:Immunoprecipitation - 1ug ATRIP antibody (A7139)}

Immunoprecipitation - 1ug ATRIP antibody (A7139)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ATRIP antibody (A7139). Western blot was performed from the immunoprecipitate using ATRIP antibody (A7139) at a dilition of 1:1000.

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