Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ATP11C Rabbit pAb (A16616)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - ATP11C Rabbit pAb (A16616)

Western blot analysis of various lysates using ATP11C Rabbit pAb (A16616) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Overview

Product name ATP11C Rabbit pAb
Catalog No. A16616
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables phosphatidylethanolamine flippase activity and phosphatidylserine flippase activity. Predicted to be involved in phospholipid translocation; positive regulation of B cell differentiation; and pre-B cell differentiation. Located in endoplasmic reticulum and plasma membrane. Is integral component of plasma membrane. Implicated in X-linked congenital hemolytic anemia.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 430-650 of human ATP11C (NP_775965.2).
Sequence DGHKYKGVTQEVDGLSQTDGTLTYFDKVDKNREELFLRALCLCHTVEIKTNDAVDGATESAELTYISSSPDEIALVKGAKRYGFTFLGNRNGYMRVENQRKEIEEYELLHTLNFDAVRRRMSVIVKTQEGDILLFCKGADSAVFPRVQNHEIELTKVHVERNAMDGYRTLCVAFKEIAPDDYERINRQLIEAKMALQDREEKMEKVFDDIETNMNLIGATA
Gene ID 286410
Swiss prot Q8NB49
Synonyms ATPIG; ATPIQ; HACXL; ATP11C
Calculated MW 129kDa
Observed MW 157kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HepG2, U-251MG, Mouse lung
Cellular location Cell membrane, Endoplasmic reticulum membrane, Multi-pass membrane protein

ATP11C Rabbit pAb images

ABclonal:Western blot - ATP11C Rabbit pAb (A16616)}

Western blot - ATP11C Rabbit pAb (A16616)

Western blot analysis of various lysates using ATP11C Rabbit pAb (A16616) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ATP11C. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ATP11C. (Distance between topics and target gene indicate popularity.) ATP11C

* Data provided by citexs.com, for reference only.

Publishing research using A16616? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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