Products > Catalog Antibodies > Monoclonal Antibodies

ATM Rabbit mAb (A19650)

Publications (4) Datasheet

ABclonal:Western blot - ATM Rabbit mAb (A19650)

Western blot analysis of extracts of various cell lines, using ATM antibody (A19650) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ATM Rabbit mAb (A19650)

Immunohistochemistry of paraffin-embedded rat spleen using ATM antibody (A19650) at dilution of 1:100 (40x lens).

Overview

Product nameATM Rabbit mAb
Catalog No.A19650
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]
ImmunogenA synthesized peptide derived from human ATM.
SequenceEmail for sequence
Gene ID472
Swiss protQ13315
SynonymsAT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO1; ATM
Calculated MW350kDa
Observed MW350KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesRaji, HepG2, HeLa, 293T, Mouse testis
Cellular location
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - ATM Rabbit mAb (A19650) }

Western blot - ATM Rabbit mAb (A19650)

Western blot analysis of extracts of various cell lines, using ATM antibody (A19650) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ATM Rabbit mAb (A19650) }

Immunohistochemistry - ATM Rabbit mAb (A19650)

Immunohistochemistry of paraffin-embedded rat spleen using ATM antibody (A19650) at dilution of 1:100 (40x lens).

* For research use only. Not for therapeutic or diagnostic purposes.

Inquire About This Product

Submit your question about A19650 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A19650? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
Contact us to order