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ATG5 Rabbit pAb (A0203)

Reviews (6)Publications (18) Datasheet

ABclonal:Western blot - ATG5 Rabbit pAb (A0203)
ABclonal:Immunohistochemistry - ATG5 Rabbit pAb (A0203)
ABclonal:Immunohistochemistry - ATG5 Rabbit pAb (A0203)
ABclonal:Immunohistochemistry - ATG5 Rabbit pAb (A0203)
ABclonal:Immunofluorescence - ATG5 Rabbit pAb (A0203)
Review
ABclonal: review for ATG5 Rabbit pAb(A0203)
Review
ABclonal: review for ATG5 Rabbit pAb(A0203)
Review
ABclonal: review for ATG5 Rabbit pAb(A0203)
Review
ABclonal: review for ATG5 Rabbit pAb(A0203)
Review
ABclonal: review for ATG5 Rabbit pAb(A0203)
Review
ABclonal: review for ATG5 Rabbit pAb(A0203)

Overview

Product nameATG5 Rabbit pAb
Catalog No.A0203
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene, in combination with autophagy protein 12, functions as an E1-like activating enzyme in a ubiquitin-like conjugating system. The encoded protein is involved in several cellular processes, including autophagic vesicle formation, mitochondrial quality control after oxidative damage, negative regulation of the innate antiviral immune response, lymphocyte development and proliferation, MHC II antigen presentation, adipocyte differentiation, and apoptosis. Several transcript variants encoding different protein isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human ATG5 (NP_004840.1).
SequenceMTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD
Gene ID9474
Swiss protQ9H1Y0
SynonymsATG5; APG5; APG5-LIKE; APG5L; ASP; hAPG5
Calculated MW22kDa/32kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, NIH/3T3, U-87MG
Cellular locationCytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane
Customer validation

(Human colon cancer cell, Human chronic myeloid leukemia cells, H460, Mouse lung tumor, A549,Mouse cells, HeLa)

WB(Homo sapiens, Sus scrofa, Gallus gallus, Columba livia, Rattus norvegicus, Mus musculus)

IF(Sus scrofa)

ABclonal:Western blot - ATG5 Rabbit pAb (A0203) }

Western blot - ATG5 Rabbit pAb (A0203)

Western blot analysis of extracts of various cell lines, using ATG5 antibody (A0203) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - ATG5 Rabbit pAb (A0203) }

Immunohistochemistry - ATG5 Rabbit pAb (A0203)

Immunohistochemistry of paraffin-embedded Mouse kidney using ATG5 Rabbit pAb (A0203) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - ATG5 Rabbit pAb (A0203) }

Immunohistochemistry - ATG5 Rabbit pAb (A0203)

Immunohistochemistry of paraffin-embedded Rat kidney using ATG5 Rabbit pAb (A0203) at dilution of 1:50 (40x lens).
ABclonal:Immunohistochemistry - ATG5 Rabbit pAb (A0203) }

Immunohistochemistry - ATG5 Rabbit pAb (A0203)

Immunohistochemistry of paraffin-embedded Human colon using ATG5 Rabbit pAb (A0203) at dilution of 1:50 (40x lens).
ABclonal:Immunofluorescence - ATG5 Rabbit pAb (A0203) }

Immunofluorescence - ATG5 Rabbit pAb (A0203)

Immunofluorescence analysis of HeLa cells using ATG5 antibody (A0203) at dilution of 1:100. Blue: DAPI for nuclear staining.

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