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ATG12 Rabbit mAb (A19610)

Publication (1) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATG12 Rabbit mAb (A19610)

Western blot analysis of extracts of various cell lines, using ATG12 antibody (A19610) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - ATG12 Rabbit mAb (A19610)

Immunohistochemistry of paraffin-embedded mouse lung using ATG12 Rabbit mAb (A19610) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunoprecipitation - ATG12 Rabbit mAb (A19610)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG12 antibody (A19610). Western blot was performed from the immunoprecipitate using ATG12 antibody (A19610) at a dilition of 1:500.

Overview

Product nameATG12 Rabbit mAb
Catalog No.A19610
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 61-140 of human Apg12 (O94817).
SequenceAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG
Gene ID9140
Swiss protO94817
SynonymsATG12; APG12; APG12L; FBR93; HAPG12; Apg12
Calculated MW22, 55kDa
Observed MW55KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
  • IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesPC-3, Mouse brain, Mouse stomach
Cellular locationCytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane
Customer validation

WB(Rattus norvegicus)

ABclonal:Western blot - ATG12 Rabbit mAb (A19610)}

Western blot - ATG12 Rabbit mAb (A19610)

Western blot analysis of extracts of various cell lines, using ATG12 antibody (A19610) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
ABclonal:Immunohistochemistry - ATG12 Rabbit mAb (A19610)}

Immunohistochemistry - ATG12 Rabbit mAb (A19610)

Immunohistochemistry of paraffin-embedded mouse lung using ATG12 Rabbit mAb (A19610) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunoprecipitation - ATG12 Rabbit mAb (A19610)}

Immunoprecipitation - ATG12 Rabbit mAb (A19610)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG12 antibody (A19610). Western blot was performed from the immunoprecipitate using ATG12 antibody (A19610) at a dilition of 1:500.

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