Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

ATG12 Mouse mAb (A8078)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - ATG12 Mouse mAb (A8078)

Western blot analysis of extracts of PC-3 cells, using ATG12 antibody (A8078).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name ATG12 Mouse mAb
Catalog No. A8078
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).

Immunogen information

Immunogen A synthetic peptide of human ATG12
Sequence Email for sequence
Gene ID 9140
Swiss prot O94817
Synonyms APG12; FBR93; APG12L; HAPG12; ATG12
Calculated MW 15kDa
Observed MW 53kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:10 - 1:100
  • IF/ICC 1:10 - 1:100
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples PC-3
Cellular location Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane

Research Area

ATG12 Mouse mAb images

ABclonal:Western blot - ATG12 Mouse mAb (A8078)}

Western blot - ATG12 Mouse mAb (A8078)

Western blot analysis of extracts of PC-3 cells, using ATG12 antibody (A8078).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on ATG12. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to ATG12. (Distance between topics and target gene indicate popularity.) ATG12

* Data provided by citexs.com, for reference only.

Publishing research using A8078? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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