Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ASC/TMS1 Rabbit pAb (A11433)

Publications (17) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - ASC/TMS1 Rabbit pAb (A11433)

Western blot analysis of extracts of Mouse spleen, using ASC/TMS1 antibody (A11433) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - ASC/TMS1 Rabbit pAb (A11433)

Western blot analysis of extracts of Rat spleen, using ASC/TMS1 antibody (A11433) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)

Immunohistochemistry analysis of paraffin-embedded human colon using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)

Immunohistochemistry analysis of paraffin-embedded human tonsil using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)

Immunohistochemistry analysis of paraffin-embedded mouse lung using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)

Immunohistochemistry analysis of paraffin-embedded rat kidney using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A11433)

Immunofluorescence analysis of BALB-3T3 cells using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A11433)

Immunofluorescence analysis of C6 cells using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A11433)

Immunofluorescence analysis of U2OS cells using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name ASC/TMS1 Rabbit pAb
Catalog No. A11433
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ASC/TMS1 (NP_037390.2).
Sequence LKLLSVPLREGYGRIPRG
Gene ID 29108
Swiss prot Q9ULZ3
Synonyms ASC; TMS; TMS1; CARD5; TMS-1; ASC/TMS1
Calculated MW 22kDa
Observed MW 19kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples Mouse spleen, Rat spleen
Cellular location Cytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus
Customer validation

IF (Mus musculus)

WB (Mus musculus, Homo sapiens, Rattus norvegicus, Canis lupus familiaris)

IHC (Mus musculus, Homo sapiens, Canis lupus familiaris)

Documents

Certificate of Compliance

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Lot number

Research Area

ASC/TMS1 Rabbit pAb images

ABclonal:Western blot - ASC/TMS1 Rabbit pAb (A11433)}

Western blot - ASC/TMS1 Rabbit pAb (A11433)

Western blot analysis of extracts of Mouse spleen, using ASC/TMS1 antibody (A11433) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - ASC/TMS1 Rabbit pAb (A11433)}

Western blot - ASC/TMS1 Rabbit pAb (A11433)

Western blot analysis of extracts of Rat spleen, using ASC/TMS1 antibody (A11433) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)}

Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)

Immunohistochemistry analysis of paraffin-embedded human colon using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)}

Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)

Immunohistochemistry analysis of paraffin-embedded human tonsil using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)}

Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)

Immunohistochemistry analysis of paraffin-embedded mouse lung using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)}

Immunohistochemistry - ASC/TMS1 Rabbit pAb (A11433)

Immunohistochemistry analysis of paraffin-embedded rat kidney using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A11433)}

Immunofluorescence - ASC/TMS1 Rabbit pAb (A11433)

Immunofluorescence analysis of BALB-3T3 cells using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A11433)}

Immunofluorescence - ASC/TMS1 Rabbit pAb (A11433)

Immunofluorescence analysis of C6 cells using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ASC/TMS1 Rabbit pAb (A11433)}

Immunofluorescence - ASC/TMS1 Rabbit pAb (A11433)

Immunofluorescence analysis of U2OS cells using ASC/TMS1 Rabbit pAb (A11433) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PYCARD. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PYCARD. (Distance between topics and target gene indicate popularity.) PYCARD

* Data provided by citexs.com, for reference only.

Publishing research using A11433? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

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Secondary Antibodies (23)

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