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ASC / TMS1 Rabbit pAb (A1170)

Reviews (2)Publications (2) Datasheet

ABclonal:Western blot - ASC / TMS1 Polyclonal Antibody (A1170)
ABclonal:Immunofluorescence - ASC / TMS1 Polyclonal Antibody (A1170)
Review
ABclonal: review for ASC / TMS1 Rabbit pAb(A1170)
Review
ABclonal: review for ASC / TMS1 Rabbit pAb(A1170)

Overview

Product nameASC / TMS1 Rabbit pAb
Catalog No.A1170
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-195 of human ASC / TMS1 (NP_037390.2).
SequenceLDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS
Gene ID29108
Swiss protQ9ULZ3
SynonymsPYCARD; ASC; CARD5; TMS; TMS-1; TMS1; PYD and CARD domain containing; ASC / TMS1
Calculated MW15kDa/19kDa/21kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesA-549, THP-1, HT-29, BT-474
Cellular locationCytoplasm, Endoplasmic reticulum, Mitochondrion, Nucleus
Customer validation

(Rat brain)

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - ASC / TMS1 Polyclonal Antibody (A1170) }

Western blot - ASC / TMS1 Polyclonal Antibody (A1170)

Western blot analysis of extracts of various cell lines, using ASC / TMS1 antibody (A1170).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - ASC / TMS1 Polyclonal Antibody (A1170) }

Immunofluorescence - ASC / TMS1 Polyclonal Antibody (A1170)

Immunofluorescence analysis of HeLa cells using ASC / TMS1 antibody (A1170).

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