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APC Rabbit pAb (A2818)

Reviews (2)Publication (1) Datasheet

ABclonal:Western blot - APC Rabbit pAb (A2818)

Western blot analysis of extracts of various cell lines, using (A2818) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - APC Rabbit pAb (A2818)

Immunohistochemistry of paraffin-embedded human lung using APC antibody (A2818) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - APC Rabbit pAb (A2818)

Immunohistochemistry of paraffin-embedded human colon using APC antibody (A2818) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - APC Rabbit pAb (A2818)

Immunohistochemistry of paraffin-embedded human breast cancer using APC antibody (A2818) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - APC Rabbit pAb (A2818)

Immunofluorescence analysis of HT-29 cells using APC antibody (A2818) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - APC Rabbit pAb (A2818)

Immunofluorescence analysis of mouse colon using APC antibody (A2818) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for APC Rabbit pAb(A2818)
Review
ABclonal: review for APC Rabbit pAb(A2818)

Overview

Product nameAPC Rabbit pAb
Catalog No.A2818
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2544-2843 of human APC (NP_000029.2).
SequenceSGTWKREHSKHSSSLPRVSTWRRTGSSSSILSASSESSEKAKSEDEKHVNSISGTKQSKENQVSAKGTWRKIKENEFSPTNSTSQTVSSGATNGAESKTLIYQMAPAVSKTEDVWVRIEDCPINNPRSGRSPTGNTPPVIDSVSEKANPNIKDSKDNQAKQNVGNGSVPMRTVGLENRLNSFIQVDAPDQKGTEIKPGQNNPVPVSETNESSIVERTPFSSSSSSKHSSPSGTVAARVTPFNYNPSPRKSSADSTSARPSQIPTPVNNNTKKRDSKTDSTESSGTQSPKRHSGSYLVTSV
Gene ID324
Swiss protP25054
SynonymsAPC; BTPS2; DP2; DP2.5; DP3; GS; PPP1R46
Calculated MW300kDa/311kDa
Observed MW150KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSW480, Mouse brain, Rat brain
Cellular locationCell junction, Cell membrane, Cell projection, Cytoplasm, adherens junction, cytoskeleton, lamellipodium, ruffle membrane
Customer validation

(Human chronic myeloid leukemia cells, HeLa cells)

ICC(Mus musculus)

ABclonal:Western blot - APC Rabbit pAb (A2818) }

Western blot - APC Rabbit pAb (A2818)

Western blot analysis of extracts of various cell lines, using (A2818) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - APC Rabbit pAb (A2818) }

Immunohistochemistry - APC Rabbit pAb (A2818)

Immunohistochemistry of paraffin-embedded human lung using APC antibody (A2818) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - APC Rabbit pAb (A2818) }

Immunohistochemistry - APC Rabbit pAb (A2818)

Immunohistochemistry of paraffin-embedded human colon using APC antibody (A2818) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - APC Rabbit pAb (A2818) }

Immunohistochemistry - APC Rabbit pAb (A2818)

Immunohistochemistry of paraffin-embedded human breast cancer using APC antibody (A2818) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - APC Rabbit pAb (A2818) }

Immunofluorescence - APC Rabbit pAb (A2818)

Immunofluorescence analysis of HT-29 cells using APC antibody (A2818) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - APC Rabbit pAb (A2818) }

Immunofluorescence - APC Rabbit pAb (A2818)

Immunofluorescence analysis of mouse colon using APC antibody (A2818) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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