Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ANXA13 Rabbit pAb (A13728)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - ANXA13 Rabbit pAb (A13728)

Western blot analysis of various lysates using ANXA13 Rabbit pAb (A13728) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ANXA13 Rabbit pAb (A13728)

Immunohistochemistry analysis of ANXA13 in paraffin-embedded human colon carcinoma using ANXA13 Rabbit pAb (A13728) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ANXA13 Rabbit pAb (A13728)

Immunohistochemistry analysis of ANXA13 in paraffin-embedded mouse jejunum using ANXA13 Rabbit pAb (A13728) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ANXA13 Rabbit pAb (A13728)

Immunohistochemistry analysis of ANXA13 in paraffin-embedded rat jejunum using ANXA13 Rabbit pAb (A13728) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name ANXA13 Rabbit pAb
Catalog No. A13728
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. The specific function of this gene has not yet been determined; however, it is associated with the plasma membrane of undifferentiated, proliferating endothelial cells and differentiated villus enterocytes. Alternatively spliced transcript variants encoding different isoforms have been identified.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human ANXA13 (NP_004297.2).
Sequence MGNRHAKASSPQGFDVDRDAKKLNKACKGMGTNEAAIIEILSGRTSDERQQIKQKYKATYGKELEEVLKSELSGNFEKTALALLDRPSEYAARQLQKAMKGLGTDESVLIEVLCTRTNKEIIAIKEAYQRLFDRSLESDVKGDTSGNLKKILVSLLQANRNEGDDVDKDLAGQDAKDLYDAGEGRWGTDELAFNEVLAKRSYKQLRATFQAYQILIGKDIEEAIEEETSGDLQKAYLTLVRCAQDCEDYFAERLYKSMKGAGTDEETLIRIVVTRAEVDLQGIKAKFQEKYQKSLSDMVRSDTSGDFRKLLVALLH
Gene ID 312
Swiss prot P27216
Synonyms ISA; ANX13; ANXA13
Calculated MW 35kDa
Observed MW 36kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples LO2, Mouse small intestine, Mouse kidney, Rat small intestine
Cellular location Cell membrane

Documents

Certificate of Compliance

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Research Area

ANXA13 Rabbit pAb images

ABclonal:Western blot - ANXA13 Rabbit pAb (A13728)}

Western blot - ANXA13 Rabbit pAb (A13728)

Western blot analysis of various lysates using ANXA13 Rabbit pAb (A13728) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ANXA13 Rabbit pAb (A13728)}

Immunohistochemistry - ANXA13 Rabbit pAb (A13728)

Immunohistochemistry analysis of ANXA13 in paraffin-embedded human colon carcinoma using ANXA13 Rabbit pAb (A13728) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ANXA13 Rabbit pAb (A13728)}

Immunohistochemistry - ANXA13 Rabbit pAb (A13728)

Immunohistochemistry analysis of ANXA13 in paraffin-embedded mouse jejunum using ANXA13 Rabbit pAb (A13728) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ANXA13 Rabbit pAb (A13728)}

Immunohistochemistry - ANXA13 Rabbit pAb (A13728)

Immunohistochemistry analysis of ANXA13 in paraffin-embedded rat jejunum using ANXA13 Rabbit pAb (A13728) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on ANXA13. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ANXA13. (Distance between topics and target gene indicate popularity.) ANXA13

* Data provided by citexs.com, for reference only.

Publishing research using A13728? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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