Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

AMPKα1 Rabbit pAb (A1229)

Reviews (3) Publications (52) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - AMPKα1 Rabbit pAb (A1229)

Western blot analysis of lysates from Rat liver, using AMPKα1 Rabbit pAb (A1229) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - AMPKα1 Rabbit pAb (A1229)

Immunofluorescence analysis of HeLa cells using AMPKα1 Rabbit pAb(A1229) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - AMPKα1 Rabbit pAb (A1229)

Immunofluorescence analysis of MCF7 cells using AMPKα1 Rabbit pAb(A1229) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - AMPKα1 Rabbit pAb (A1229)

Immunofluorescence analysis of PC-12 cells using AMPKα1 Rabbit pAb(A1229) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for AMPKα1 Rabbit pAb(A1229)
Review
ABclonal: review for AMPKα1 Rabbit pAb(A1229)
Review
ABclonal: review for AMPKα1 Rabbit pAb(A1229)

You may also interested in:

Overview

Product name AMPKα1 Rabbit pAb
Catalog No. A1229
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 381-520 of human AMPKα1 (NP_006242.5).
Sequence Email for sequence
Gene ID 5562
Swiss prot Q13131
Synonyms AMPK; AMPKa1; AMPK alpha 1; AMPKα1
Calculated MW 64kDa
Observed MW 64kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Rat liver
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Human lung cancer cells, HeLaHomo sapiens, Rattus norvegicus, Homo sapiens, Mus musculus, Danio rerio, Sus scrofa, Gallus gallus, Ctenopharyngodon idellus, Arabidopsis thaliana)

IF (Human lung cancer cellsMus musculus, Ctenopharyngodon idellus)

IHC (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

AMPKα1 Rabbit pAb images

ABclonal:Western blot - AMPKα1 Rabbit pAb (A1229)}

Western blot - AMPKα1 Rabbit pAb (A1229)

Western blot analysis of lysates from Rat liver, using AMPKα1 Rabbit pAb (A1229) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - AMPKα1 Rabbit pAb (A1229)}

Immunofluorescence - AMPKα1 Rabbit pAb (A1229)

Immunofluorescence analysis of HeLa cells using AMPKα1 Rabbit pAb(A1229) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - AMPKα1 Rabbit pAb (A1229)}

Immunofluorescence - AMPKα1 Rabbit pAb (A1229)

Immunofluorescence analysis of MCF7 cells using AMPKα1 Rabbit pAb(A1229) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - AMPKα1 Rabbit pAb (A1229)}

Immunofluorescence - AMPKα1 Rabbit pAb (A1229)

Immunofluorescence analysis of PC-12 cells using AMPKα1 Rabbit pAb(A1229) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Submit an review for A1229

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A1229 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on PRKAA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PRKAA1. (Distance between topics and target gene indicate popularity.) PRKAA1

* Data provided by citexs.com, for reference only.

Publishing research using A1229? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order