Reactivity:Human, Mouse, Rat
Western blot analysis of various lysates, using AMACR antibody (A20825) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human kidney using AMACR Rabbit mAb (A20825) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
|Product name||AMACR Rabbit mAb|
|Purification method||Affinity purification|
|Immunogen||Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human AMACR (NP_055139.4).|
|Synonyms||RM; RACE; CBAS4; P504S; AMACRD; AMACR|
|Reactivity||Human, Mouse, Rat|
|Tested applications||Testing results|
|Storage buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
|Application key||Western blotting Immunohistochemistry|
|Positive samples||LNCaP, Mouse liver, Mouse kidney, Rat liver, Rat kidney|
|Cellular location||Mitochondrion, Peroxisome|
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Based on existing publications, the following genes are closely related to research on AMACR. (Numbers indicate number of published articles where both targets appear.)
Based on existing publications, the following research topics are related to AMACR. (Distance between topics and target gene indicate popularity.) AMACR
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* For research use only. Not for therapeutic or diagnostic purposes.