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ALDH1L1 Rabbit pAb (A7067)

Publication (1) Datasheet

ABclonal:Western blot - ALDH1L1 Polyclonal Antibody (A7067)

Western blot analysis of extracts of various cell lines, using ALDH1L1 antibody (A7067) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameALDH1L1 Rabbit pAb
Catalog No.A7067
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ALDH1L1 (NP_036322.2).
SequenceMKIAVIGQSLFGQEVYCHLRKEGHEVVGVFTVPDKDGKADPLGLEAEKDGVPVFKYSRWRAKGQALPDVVAKYQALGAELNVLPFCSQFIPMEIISAPRHGSIIYHPSLLPRHRGASAINWTLIHGDKKGGFSIFWADDGLDTGDLLLQKECEVLPDDTVSTLYNRFLFPEGIKGMVQAVRLIAEGKAPRLPQPEEGATYEGIQKKETAKINWDQPAEAIHNWIRGNDKVPGAWTEACEQKLTFFNSTLN
Gene ID10840
Swiss protO75891
SynonymsALDH1L1; 10-FTHFDH; 10-fTHF; FDH; FTHFD
Calculated MW55kDa/87kDa/98kDa/99kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHepG2, MCF7, Jurkat, Mouse liver, Mouse brain, Mouse heart, Mouse kidney, Rat brain, Rat liver
Cellular locationCytoplasm
Customer validation

IF(Rattus norvegicus)

ABclonal:Western blot - ALDH1L1 Polyclonal Antibody (A7067) }

Western blot - ALDH1L1 Polyclonal Antibody (A7067)

Western blot analysis of extracts of various cell lines, using ALDH1L1 antibody (A7067) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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