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ALDH1A1 Polyclonal Antibody (A1802)

Publication (3) Datasheet

ABclonal:Western blot - ALDH1A1 Polyclonal Antibody (A1802)
ABclonal:Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802)
ABclonal:Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802)
ABclonal:Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802)

Overview

Product nameALDH1A1 Polyclonal Antibody
Catalog No.A1802
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 48-147 of human ALDH1A1 (NP_000680.2).
SequenceELCQVEEGDKEDVDKAVKAARQAFQIGSPWRTMDASERGRLLYKLADLIERDRLLLATMESMNGGKLYSNAYLNDLAGCIKTLRYCAGWADKIQGRTIPI
Gene ID216
Swiss protP00352
SynonymsALDH1A1; ALDC; ALDH-E1; ALDH1; ALDH11; HEL-9; HEL-S-53e; HEL12; PUMB1; RALDH1
Calculated MW54kDa
Observed MW50-55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesA-549, HT-29, K-562, Mouse liver, Mouse lung, Rat lung
Cellular locationCytoplasm
Customer validation

WB(Human)

ABclonal:Western blot - ALDH1A1 Polyclonal Antibody (A1802) }

Western blot - ALDH1A1 Polyclonal Antibody (A1802)

Western blot analysis of extracts of various cell lines, using ALDH1A1 antibody (A1802) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802) }

Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802)

Immunohistochemistry of paraffin-embedded rat liver using ALDH1A1 antibody (A1802) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802) }

Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802)

Immunohistochemistry of paraffin-embedded human colon carcinoma using ALDH1A1 antibody (A1802) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802) }

Immunohistochemistry - ALDH1A1 Polyclonal Antibody (A1802)

Immunohistochemistry of paraffin-embedded mouse brain using ALDH1A1 antibody (A1802) at dilution of 1:100 (40x lens).

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