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AKT2 Rabbit pAb (A0336)

Publications (3) Datasheet

ABclonal:Western blot - AKT2 Rabbit pAb (A0336)

Western blot analysis of extracts of various cell lines, using AKT2 antibody (A0336).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - AKT2 Rabbit pAb (A0336)

Immunofluorescence analysis of HeLa cells using AKT2 antibody (A0336) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameAKT2 Rabbit pAb
Catalog No.A0336
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
ImmunogenA synthetic peptide of human AKT2
SequenceEmail for sequence
Gene ID208
Swiss protP31751
SynonymsAKT2; HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA
Calculated MW51kDa/55kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, NIH/3T3
Cellular locationCell membrane, Cytoplasm, Early endosome, Nucleus, Peripheral membrane protein
Customer validation

WB(Mus musculus, Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - AKT2 Rabbit pAb (A0336) }

Western blot - AKT2 Rabbit pAb (A0336)

Western blot analysis of extracts of various cell lines, using AKT2 antibody (A0336).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - AKT2 Rabbit pAb (A0336) }

Immunofluorescence - AKT2 Rabbit pAb (A0336)

Immunofluorescence analysis of HeLa cells using AKT2 antibody (A0336) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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