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AKT1 Rabbit pAb (A11016)

Review (1)Publications (43) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - AKT1 Rabbit pAb (A11016)

Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (A11016) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - AKT1 Rabbit pAb (A11016)

Immunohistochemistry of paraffin-embedded human mammary cancer using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - AKT1 Rabbit pAb (A11016)

Immunohistochemistry of paraffin-embedded human uterine cancer using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - AKT1 Rabbit pAb (A11016)

Immunohistochemistry of paraffin-embedded human tonsil using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - AKT1 Rabbit pAb (A11016)

Immunohistochemistry of paraffin-embedded human lung cancer using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - AKT1 Rabbit pAb (A11016)

Immunofluorescence analysis of L929 cells using AKT1 antibody (A11016) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for AKT1 Rabbit pAb(A11016)

Overview

Product nameAKT1 Rabbit pAb
Catalog No.A11016
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 381-480 of human AKT1 (NP_005154.2).
SequenceSGLLKKDPKQRLGGGSEDAKEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA
Gene ID207
Swiss protP31749
SynonymsAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1
Calculated MW48kDa/55kDa
Observed MW60KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMCF7
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

(rat cells)

IHC(Rattus norvegicus)

WB(Rattus norvegicus, Homo sapiens, Mus musculus, Cyprinus carpio, Dendrobium officinale Kimura et Migo, Capra hircus, Gallus gallus, Ctenopharyngodon idellus, Apocynum venetum leaf)

IF(Rattus norvegicus)

Co-IP(Homo sapiens)

ABclonal:Western blot - AKT1 Rabbit pAb (A11016) }

Western blot - AKT1 Rabbit pAb (A11016)

Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (A11016) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - AKT1 Rabbit pAb (A11016) }

Immunohistochemistry - AKT1 Rabbit pAb (A11016)

Immunohistochemistry of paraffin-embedded human mammary cancer using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - AKT1 Rabbit pAb (A11016) }

Immunohistochemistry - AKT1 Rabbit pAb (A11016)

Immunohistochemistry of paraffin-embedded human uterine cancer using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - AKT1 Rabbit pAb (A11016) }

Immunohistochemistry - AKT1 Rabbit pAb (A11016)

Immunohistochemistry of paraffin-embedded human tonsil using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - AKT1 Rabbit pAb (A11016) }

Immunohistochemistry - AKT1 Rabbit pAb (A11016)

Immunohistochemistry of paraffin-embedded human lung cancer using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - AKT1 Rabbit pAb (A11016) }

Immunofluorescence - AKT1 Rabbit pAb (A11016)

Immunofluorescence analysis of L929 cells using AKT1 antibody (A11016) at dilution of 1:100. Blue: DAPI for nuclear staining.

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