Review (1)Publications (29) Datasheet
Western blot analysis of extracts of MCF7 cells, using AKT1 antibody (A11016) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human mammary cancer using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using AKT1 antibody (A11016) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of L929 cells using AKT1 antibody (A11016) at dilution of 1:100. Blue: DAPI for nuclear staining.
| Product name | AKT1 Rabbit pAb |
|---|---|
| Catalog No. | A11016 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of human AKT1 (NP_005154.2). |
|---|---|
| Sequence | KEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSMECVDSERRPHFPQFSYSASGTA |
| Gene ID | 207 |
| Swiss prot | P31749 |
| Synonyms | AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1 |
| Calculated MW | 48kDa/55kDa |
| Observed MW | 60KDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:200 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Application key | Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation |
| Positive samples | MCF7 |
| Cellular location | Cell membrane, Cytoplasm, Nucleus |
| Customer validation | (rat cells) WB(Mus musculus, Homo sapiens, Cyprinus carpio, Dendrobium officinale Kimura et Migo, Capra hircus, Gallus gallus, Ctenopharyngodon idellus, Rattus norvegicus) IHC(Rattus norvegicus) IF(Rattus norvegicus) Co-IP(Homo sapiens) |
* For research use only. Not for therapeutic or diagnostic purposes.
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