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AKT1 Rabbit pAb (A7270)

Publications (7) Datasheet

ABclonal:Western blot - AKT1 Rabbit pAb (A7270)

Overview

Product nameAKT1 Rabbit pAb
Catalog No.A7270
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human AKT1 (NP_005154.2).
SequenceAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEF
Gene ID207
Swiss protP31749
SynonymsAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA; AKT1
Calculated MW48kDa/55kDa
Observed MW57kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesNCI-H460, MCF7, Mouse kidney, Rat heart
Cellular locationCell membrane, Cytoplasm, Nucleus
Customer validation

WB(Rattus norvegicus, Homo sapiens)

ABclonal:Western blot - AKT1 Rabbit pAb (A7270) }

Western blot - AKT1 Rabbit pAb (A7270)

Western blot analysis of extracts of various cell lines, using AKT1 antibody (A7270).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

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