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AFP Mouse mAb (A11865)

Reviews (2) Datasheet

ABclonal:Western blot - AFP Monoclonal Antibody (A11865)
ABclonal:Immunohistochemistry - AFP Monoclonal Antibody (A11865)
ABclonal:Immunofluorescence - AFP Monoclonal Antibody (A11865)
Review
ABclonal: review for AFP Mouse mAb(A11865)
Review
ABclonal: review for AFP Mouse mAb(A11865)

Overview

Product nameAFP Mouse mAb
Catalog No.A11865
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
ImmunogenRecombinant protein of human AFP
SequenceEmail for sequence
Gene ID174
Swiss protP02771
SynonymsAFPD; FETA; HPAFP; AFP
Calculated MW68kDa
Observed MW69kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, human plasma, SH-SY5Y, mouse plasma, mouse liver, mouse kidney, rat plasma, rat liver
Cellular locationSecreted
Customer validation

(Human colon cancer cell)

ABclonal:Western blot - AFP Monoclonal Antibody (A11865) }

Western blot - AFP Monoclonal Antibody (A11865)

Western blot analysis of extracts of various cells, using AFP antibody (A11865) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunohistochemistry - AFP Monoclonal Antibody (A11865) }

Immunohistochemistry - AFP Monoclonal Antibody (A11865)

Immunohistochemistry of paraffin-embedded human liver cancer using AFP antibody (A11865) at dilution of 1:12.5 (40x lens).
ABclonal:Immunofluorescence - AFP Monoclonal Antibody (A11865) }

Immunofluorescence - AFP Monoclonal Antibody (A11865)

Immunofluorescence analysis of human liver cancer using AFP antibody (A11865) at dilution of 1:100. Blue: DAPI for nuclear staining.

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