Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ADAM28 Rabbit pAb (A14849)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - ADAM28 Rabbit pAb (A14849)

Western blot analysis of extracts of various cell lines, using ADAM28 Rabbit pAb (A14849) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Overview

Product name ADAM28 Rabbit pAb
Catalog No. A14849
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. This gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 199-440 of human ADAM28 (NP_055080.2).
Sequence VQEHEKYIEYYLVLDNGEFKRYNENQDEIRKRVFEMANYVNMLYKKLNTHVALVGMEIWTDKDKIKITPNASFTLENFSKWRGSVLSRRKRHDIAQLITATELAGTTVGLAFMSTMCSPYSVGVVQDHSDNLLRVAGTMAHEMGHNFGMFHDDYSCKCPSTICVMDKALSFYIPTDFSSCSRLSYDKFFEDKLSNCLFNAPLPTDIISTPICGNQLVEMGEDCDCGTSEECTNICCDAKTCK
Gene ID 10863
Swiss prot Q9UKQ2
Synonyms MDCL; MDC-L; eMDCII; ADAM 28; eMDC II; ADAM28
Calculated MW 87kDa
Observed MW 87kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples SGC-7901, HT-29, THP-1, Mouse liver
Cellular location Cell membrane, Secreted, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Lot number

Research Area

ADAM28 Rabbit pAb images

ABclonal:Western blot - ADAM28 Rabbit pAb (A14849)}

Western blot - ADAM28 Rabbit pAb (A14849)

Western blot analysis of extracts of various cell lines, using ADAM28 Rabbit pAb (A14849) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ADAM28. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ADAM28. (Distance between topics and target gene indicate popularity.) ADAM28

* Data provided by citexs.com, for reference only.

Publishing research using A14849? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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