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ACTB Rabbit mAb (AC038)

Publication (1) Datasheet

ABclonal:Western blot - ACTB Rabbit mAb (AC038)
ABclonal:Western blot - ACTB Rabbit mAb (AC038)

Overview

Product nameACTB Rabbit mAb
Catalog No.AC038
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. |Put 5ul AC038 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
ImmunogenRecombinant protein of human β-actin
SequenceEmail for sequence
Gene ID60
Swiss protP60709
SynonymsACTB; BRWS1; PS1TP5BP1; beta actin; actin
Calculated MW41kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:10000 - 1:100000
IHC 1:20 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, HeLa, Mouse brain, Mouse testis, Mouse lung, Rat brain, Rat testis, Rat lung
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Mus musculus)

ABclonal:Western blot - ACTB Rabbit mAb (AC038) }

Western blot - ACTB Rabbit mAb (AC038)

Western blot analysis of extracts of various cell lines, using ACTB antibody (AC038) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - ACTB Rabbit mAb (AC038) }

Western blot - ACTB Rabbit mAb (AC038)

Western blot analysis of extracts of HeLa cells, using ACTB antibody (AC038) at 1:10000-1:320000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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