Product Type > Antibodies > Tag and Loading Control Antibodies

β-Actin Rabbit pAb (AC006)

Review (1) Publications (111) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:β-Actin Rabbit pAb
ABclonal:Western blot - β-Actin Rabbit pAb (AC006)

Western blot analysis of various lysates using β-Actin Rabbit pAb (AC006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Review
ABclonal: review for β-Actin Rabbit pAb(AC006)

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Overview

Product name β-Actin Rabbit pAb
Catalog No. AC006
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human β-Actin (NP_001092.1).
Sequence MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Gene ID 60
Swiss prot P60709
Synonyms BRWS1; PS1TP5BP1; β-Actin
Calculated MW 42kDa
Observed MW 42kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, MCF7, A-431, NIH/3T3, C6, Mouse testis, Mouse kidney, Rat testis
Cellular location Cytoplasm, cytoskeleton
Customer validation

WB (MSC cellsHomo sapiens, Crassostrea gigas, Homo sapiens, Rattus norvegicus, Mus musculus, Sus scrofa, Arabidopsis thaliana, Gallus gallus, flounder, lamprey, Melodinus cochinchinensis, Tree, Flowers, fish, acyg, Verticillium dahliae, Mouse)

IF (Homo sapiens, Rattus norvegicus, Mus musculus)

IP (Homo sapiens, Mus musculus)

qRT-PCR (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

β-Actin Rabbit pAb images

ABclonal:Western blot - β-Actin Rabbit pAb (AC006)}

Western blot - β-Actin Rabbit pAb (AC006)

Western blot analysis of various lysates using β-Actin Rabbit pAb (AC006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ACTB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ACTB. (Distance between topics and target gene indicate popularity.) ACTB

* Data provided by citexs.com, for reference only.

Publishing research using AC006? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (22)

* For research use only. Not for therapeutic or diagnostic purposes.

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