Product Type > Antibodies > Tag and Loading Control Antibodies

β-Actin Rabbit pAb (AC006)

Review (1)Publications (80) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:β-Actin Rabbit pAb
ABclonal:Western blot - β-Actin Rabbit pAb (AC006)

Western blot analysis of extracts of various cell lines, using β-Actin antibody (AC006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Review
ABclonal: review for β-Actin Rabbit pAb(AC006)

Overview

Product nameβ-Actin Rabbit pAb
Catalog No.AC006
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human β-Actin (NP_001092.1).
SequenceMDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Gene ID60
Swiss protP60709
SynonymsACTB; BRWS1; PS1TP5BP1; beta actin; actin
Calculated MW41kDa
Observed MW42kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    
Positive samplesHeLa, MCF7, A-431, NIH/3T3, C6, Mouse testis, Mouse kidney, Rat testis
Cellular locationCytoplasm, cytoskeleton
Customer validation

(MSC cells)

WB(Homo sapiens, Crassostrea gigas, Rattus norvegicus, Mus musculus, Sus scrofa, Arabidopsis thaliana, Gallus gallus, flounder, lamprey, Melodinus cochinchinensis, Tree, Flowers, fish, acyg)

IF(Homo sapiens,Rattus norvegicus, Homo sapiens, Mus musculus)

IP(Homo sapiens)

IP(Mus musculus)

Research Area

β-Actin Rabbit pAb images

ABclonal:Western blot - β-Actin Rabbit pAb (AC006)}

Western blot - β-Actin Rabbit pAb (AC006)

Western blot analysis of extracts of various cell lines, using β-Actin antibody (AC006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ACTB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ACTB. (Distance between topics and target gene indicate popularity.) ACTB

* Data provided by citexs.com, for reference only.

Publishing research using AC006? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Secondary Antibodies (26)

* For research use only. Not for therapeutic or diagnostic purposes.

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