Product Type > Antibodies > Tag and Loading Control Antibodies

β-Actin Rabbit pAb (AC006)

Review (1)Publications (80) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:β-Actin Rabbit pAb
ABclonal:Western blot - β-Actin Rabbit pAb (AC006)

Western blot analysis of extracts of various cell lines, using β-Actin antibody (AC006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Review
ABclonal: review for β-Actin Rabbit pAb(AC006)

Overview

Product nameβ-Actin Rabbit pAb
Catalog No.AC006
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human β-Actin (NP_001092.1).
SequenceMDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Gene ID60
Swiss protP60709
SynonymsACTB; BRWS1; PS1TP5BP1; beta actin; actin
Calculated MW41kDa
Observed MW42kDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thiomersal, 50% glycerol, pH7.3. May contain BSA. If BSA-free batch required, please contact us.
Application keyWestern blotting    
Positive samplesHeLa, MCF7, A-431, NIH/3T3, C6, Mouse testis, Mouse kidney, Rat testis
Cellular locationCytoplasm, cytoskeleton
Customer validation

(MSC cells)

WB(Homo sapiens, Crassostrea gigas, Rattus norvegicus, Mus musculus, Sus scrofa, Arabidopsis thaliana, Gallus gallus, flounder, lamprey, Melodinus cochinchinensis, Tree, Flowers, fish, acyg)

IF(Homo sapiens,Rattus norvegicus, Homo sapiens, Mus musculus)

IP(Homo sapiens)

IP(Mus musculus)

Research Area

β-Actin Rabbit pAb images

ABclonal:Western blot - β-Actin Rabbit pAb (AC006)}

Western blot - β-Actin Rabbit pAb (AC006)

Western blot analysis of extracts of various cell lines, using β-Actin antibody (AC006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ACTB. (Numbers indicate number of published articles where both targets appear.)

◀1/▶

Related Research Area

Based on existing publications, the following research topics are related to ACTB. (Distance between topics and target gene indicate popularity.) ACTB

* Data provided by citexs.com, for reference only.

Publishing research using AC006? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

β-Actin Rabbit mAb (High Dilution)
AC026

WB

Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

β-Actin Rabbit mAb (High Dilution)

β-Actin Mouse mAb
AC004

WBIF/ICC

Reactivity:Human, Mouse, Rat

β-Actin Mouse mAb

β-Actin Rabbit mAb
AC038

WB

Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

β-Actin Rabbit mAb

HRP-conjugated β-Actin Rabbit mAb
AC028

WB

Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

HRP-conjugated β-Actin Rabbit mAb

HRP-conjugated β-Actin Mouse mAb
AC043

WB

Reactivity:Human, Mouse, Rat

HRP-conjugated β-Actin Mouse mAb

β-Actin Rabbit mAb
AC048

WBIHC

Reactivity:Human, Mouse, Rat

β-Actin Rabbit mAb

Secondary Antibodies (26)

HRP Goat Anti-Rabbit IgG (H+L)
AS014

ELISAWBIHC

HRP Goat Anti-Rabbit IgG (H+L)

FITC Goat Anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC Goat Anti-Rabbit IgG (H+L)

Cy3 Goat Anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3 Goat Anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L)
AS053

IF/ICCFC

ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L)

HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain
AS061

WB

HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain

ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L)
AS039

IF/ICCFC

ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L)

HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain
AS063

WB

HRP-conjugated Goat Anti-Rabbit IgG Heavy Chain

Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L)
AS040

IHCIF/ICCFC

Rhodamine (TRITC) Goat Anti-Rabbit IgG (H+L)

HRP Donkey Anti-Rabbit IgG (H+L)
AS038

WBIHC

HRP Donkey Anti-Rabbit IgG (H+L)

ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L)
AS035

IF/ICCFC

ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L)

ABflo® 647-conjugated Goat Anti-Rabbit IgG (H+L)
AS060

IF/ICCFC

ABflo® 647-conjugated Goat Anti-Rabbit IgG (H+L)

Goat Anti-Rabbit IgG(H+L)
AS070

Goat Anti-Rabbit IgG(H+L)

ABflo® 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
AS073

IF/ICCFC

ABflo® 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)

ABflo® 555-conjugated Goat Anti-Rabbit IgG (H+L)
AS058

IF/ICCFC

ABflo® 555-conjugated Goat Anti-Rabbit IgG (H+L)

ABflo® 405-conjugated Goat Anti-Rabbit IgG (H+L)
AS056

IF/ICCFC

ABflo® 405-conjugated Goat Anti-Rabbit IgG (H+L)

AP Goat Anti-Rabbit IgG (H+L)
AS009

WB

AP Goat Anti-Rabbit IgG (H+L)

FITC Donkey Anti-Rabbit IgG (H+L)
AS042

IF/ICCFC

FITC Donkey Anti-Rabbit IgG (H+L)

ABflo® 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
AS074

FCIF/ICC

ABflo® 594-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)

ABflo® 647-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)
AS075

IF/ICCFC

ABflo® 647-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L)

ABflo® 750-conjugated Goat Anti-Rabbit IgG (H+L)
AS072

IF/ICCFC

ABflo® 750-conjugated Goat Anti-Rabbit IgG (H+L)

HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG (H+L)
AS079

WB

HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG (H+L)

Rabbit Anti-Chicken IgY (H+L)
AS082

WB

Rabbit Anti-Chicken IgY (H+L)

FITC AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific
AS083

IF/ICCFC

FITC AffiniPure F(ab')₂ Fragment Goat Anti-Rabbit IgG, Fc fragment specific

Rabbit Anti-Chicken IgY Fab
AS088

WB

Rabbit Anti-Chicken IgY Fab

Rabbit Anti-Chicken IgY (H+L)
AS090

WB

Rabbit Anti-Chicken IgY (H+L)

Rabbit Anti-Chicken IgY Fc
AS089

WB

Rabbit Anti-Chicken IgY Fc

* For research use only. Not for therapeutic or diagnostic purposes.

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Related Products

Antibodies (6)

β-Actin Rabbit mAb (High Dilution)
AC026

WB

Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

β-Actin Rabbit mAb (High Dilution)

β-Actin Mouse mAb
AC004

WBIF/ICC

Reactivity:Human, Mouse, Rat

β-Actin Mouse mAb

β-Actin Rabbit mAb
AC038

WB

Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

β-Actin Rabbit mAb

Secondary Antibodies (26)

HRP Goat Anti-Rabbit IgG (H+L)
AS014

ELISAWBIHC

HRP Goat Anti-Rabbit IgG (H+L)

FITC Goat Anti-Rabbit IgG (H+L)
AS011

IF/ICCFC

FITC Goat Anti-Rabbit IgG (H+L)

Cy3 Goat Anti-Rabbit IgG (H+L)
AS007

IF/ICCFC

Cy3 Goat Anti-Rabbit IgG (H+L)
View All Related Products
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