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ACTB Polyclonal Antibody (AC006)

Publications (9) Datasheet

ABclonal:Western blot - ACTB Polyclonal Antibody (AC006)
ABclonal:Immunofluorescence - ACTB Polyclonal Antibody (AC006)

Overview

Product nameACTB Polyclonal Antibody
Catalog No.AC006
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-375 of human ACTB (NP_001092.1).
SequenceMDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Gene ID60
Swiss protP60709
SynonymsACTB; BRWS1; PS1TP5BP1; actin beta
Calculated MW41kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesTHP-1, 293T, A-431, Mouse lung, Mouse lung, Mouse spleen, Mouse kidney, Mouse thymus
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Human, C. gigas, Rat, human, Rat)

IF(human,Rat)

ABclonal:Western blot - ACTB Polyclonal Antibody (AC006) }

Western blot - ACTB Polyclonal Antibody (AC006)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC006) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - ACTB Polyclonal Antibody (AC006) }

Immunofluorescence - ACTB Polyclonal Antibody (AC006)

Immunofluorescence analysis of HeLa cells using ACTB antibody (AC006) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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