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β-actin Mouse mAb (AC004)

Reviews (2)Publications (156) Datasheet

ABclonal:β-actin Mouse mAb
ABclonal:Western blot - β-actin Mouse mAb (AC004)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC004) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - β-actin Mouse mAb (AC004)

Immunohistochemistry of paraffin-embedded rat spleen using β-actin antibody (AC004) at dilution of 1:500 (40x lens).

ABclonal:Immunohistochemistry - β-actin Mouse mAb (AC004)

Immunohistochemistry of paraffin-embedded human stomach using β-actin antibody (AC004) at dilution of 1:500 (40x lens).

ABclonal:Immunohistochemistry - β-actin Mouse mAb (AC004)

Immunohistochemistry of paraffin-embedded mouse spleen using β-actin antibody (AC004) at dilution of 1:500 (40x lens).

ABclonal:Immunofluorescence - β-actin Mouse mAb (AC004)

Immunofluorescence analysis of C6 cells using β-actin Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-actin Mouse mAb (AC004)

Immunofluorescence analysis of HeLa cells using β-actin Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-actin Mouse mAb (AC004)

Immunofluorescence analysis of L929 cells using β-actin Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - β-actin Mouse mAb (AC004)

Confocal Immunofluorescent analysis of U2OS cells using KRR1 Rabbit pAb (A4487) at dilution of 1:100 (40x lens)(red). β-Actin (AC004: β-actin Mouse mAb) for Cytoskeleton staining(green), DAPI for nuclear staining(blue).

ABclonal:Immunoprecipitation - β-actin Mouse mAb (AC004)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug β-actin antibody (AC004). Western blot was performed from the immunoprecipitate using β-actin antibody (AC004) at a dilition of 1:1000.

Review
ABclonal: review for β-actin Mouse mAb(AC004)
Review
ABclonal: review for β-actin Mouse mAb(AC004)

Overview

Product nameβ-actin Mouse mAb
Catalog No.AC004
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
ImmunogenFusion protein of human β-actin
SequenceEmail for sequence
Gene ID60
Swiss protP60709
SynonymsACTB; BRWS1; PS1TP5BP1; beta actin; actin
Calculated MW41kDa
Observed MW42KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:10000 - 1:100000
IHC 1:100- 1:200
IF 1:50- 1:200
IP 1:50 - 1:200
Antibody Dilute ProcedurePut 5ul AC004 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesRD, HeLa, A-549, NIH/3T3, Mouse lung, Mouse brain, Rat testis
Cellular locationCytoplasm, cytoskeleton
Customer validation

(Mouse kidney, Rat kidney, Homo sapiens)

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Citrus sinensis, Sus scrofa, Ctenopharyngodon idellus, Crassostrea gigas, Cynoglossus semilaevis, Pinellia ternata, Spodoptera frugiperda)

IF(Homo sapiens, Mus musculus)

IP(Homo sapiens)

IF(Homo sapiens)

IHC(Homo sapiens)

IP(Homo sapiens)

WB(Homo sapiens)

IHC(Mus musculus)

IP(H)

ABclonal:Western blot - β-actin Mouse mAb (AC004) }

Western blot - β-actin Mouse mAb (AC004)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC004) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - β-actin Mouse mAb (AC004) }

Immunohistochemistry - β-actin Mouse mAb (AC004)

Immunohistochemistry of paraffin-embedded rat spleen using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - β-actin Mouse mAb (AC004) }

Immunohistochemistry - β-actin Mouse mAb (AC004)

Immunohistochemistry of paraffin-embedded human stomach using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - β-actin Mouse mAb (AC004) }

Immunohistochemistry - β-actin Mouse mAb (AC004)

Immunohistochemistry of paraffin-embedded mouse spleen using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunofluorescence - β-actin Mouse mAb (AC004) }

Immunofluorescence - β-actin Mouse mAb (AC004)

Immunofluorescence analysis of C6 cells using β-actin Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-actin Mouse mAb (AC004) }

Immunofluorescence - β-actin Mouse mAb (AC004)

Immunofluorescence analysis of HeLa cells using β-actin Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-actin Mouse mAb (AC004) }

Immunofluorescence - β-actin Mouse mAb (AC004)

Immunofluorescence analysis of L929 cells using β-actin Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - β-actin Mouse mAb (AC004) }

Immunofluorescence - β-actin Mouse mAb (AC004)

Confocal Immunofluorescent analysis of U2OS cells using KRR1 Rabbit pAb (A4487) at dilution of 1:100 (40x lens)(red). β-Actin (AC004: β-actin Mouse mAb) for Cytoskeleton staining(green), DAPI for nuclear staining(blue).
ABclonal:Immunoprecipitation - β-actin Mouse mAb (AC004) }

Immunoprecipitation - β-actin Mouse mAb (AC004)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug β-actin antibody (AC004). Western blot was performed from the immunoprecipitate using β-actin antibody (AC004) at a dilition of 1:1000.

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