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ACTB Monoclonal Antibody (AC026)

Reviews (3)Publications (9) Datasheet

ABclonal:Western blot - ACTB Monoclonal Antibody (AC026)
ABclonal:Western blot - ACTB Monoclonal Antibody (AC026)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026)
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC026)

Overview

Product nameACTB Monoclonal Antibody
Catalog No.AC026
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
ImmunogenRecombinant protein of human β-actin
SequenceEmail for sequence
Gene ID60
Swiss protP60709
SynonymsBRWS1; PS1TP5BP1
Calculated MW41kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat, Chicken, Zebrafish, Pig, Oryza sativa
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:50000 - 1:200000
IHC 1:200 - 1:2000
IF 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3, TE-1, 293T, SW480, Mouse brain, Mouse testis, Mouse lung, Rat brain, HeLa
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Pig lung, 293T, DF-1, Rat, Clam, Human, Chicken, Mouse)

CoIP(Human)

WB(Human)

ABclonal:Western blot - ACTB Monoclonal Antibody (AC026) }

Western blot - ACTB Monoclonal Antibody (AC026)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC026) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - ACTB Monoclonal Antibody (AC026) }

Western blot - ACTB Monoclonal Antibody (AC026)

Western blot analysis of extracts of HeLa cells, using β-actin antibody (AC026) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC026)

Immunohistochemistry of paraffin-embedded human breast cancer using β-actin antibody (AC026) at dilution of 1:100 (20x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC026)

Immunohistochemistry of paraffin-embedded mouse liver using β-actin antibody (AC026) at dilution of 1:100 (20x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC026) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC026)

Immunohistochemistry of paraffin-embedded rat kidney using β-actin antibody (AC026) at dilution of 1:100 (20x lens).
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC026) }

Immunofluorescence - ACTB Monoclonal Antibody (AC026)

Immunofluorescence analysis of HeLa cells using ACTB antibody (AC026) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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