Product Type > Antibodies > Tag and Loading Control Antibodies

β-Actin Rabbit mAb (High Dilution) (AC026)

Reviews (5) Publications (1888) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Chicken, Zebrafish, Pig

ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of various lysates using β-Actin Rabbit mAb (High Dilution) (AC026) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of lysates from HeLa cells, using β-Actin Rabbit mAb (High Dilution) (AC026) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)
Review
ABclonal: review for β-Actin Rabbit mAb (High Dilution)(AC026)

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Overview

Product name β-Actin Rabbit mAb (High Dilution)
Catalog No. AC026
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC5115-01

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome.
Note:Due to the high antibody titer, it is advisable to be diluted before use. Please dilute 5μL of the antibody solution with 45μL of PBS solution, containing 50% glycerol. The diluted antibody can be stored at -20°C.

Immunogen information

Immunogen Recombinant protein of human β-actin
Sequence MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF
Gene ID 60
Swiss prot P60709
Synonyms BRWS1; PS1TP5BP1; β-Actin
Calculated MW 42kDa
Observed MW 42kDa

Applications

Reactivity Human, Mouse, Rat, Chicken, Zebrafish, Pig
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:50000 - 1:200000
Antibody Dilute Procedure Put 5ul AC026 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples TE-1, SW480, NIH/3T3, 293T, Mouse brain, Mouse testis, mose lung, rat brain
Cellular location Cytoplasm, cytoskeleton
Customer validation

(HeLa treated by Starvatio and IGF)

WB (rat brain cells, Pig lung, 293T, DF-1Mus musculus, Rattus norvegicus, Mactra, Gallus gallus, Homo sapiens, Mus musculus, Bos taurus, Sus scrofa, Danio rerio, Zea mays, Cynoglossus semilaevis, Mycobacterium tuberculosis, Cavia porcellus, Macaca mulatta, Arabidopsis thaliana, Squaliobarbus curriculus, Malus micromalus, Cyprinus carpio, Alpinia katsumadai Hayata, Helicoverpa armigera, Anatinae, Oreochroms mossambcus, Cricetinae, Edwardsiella tarda, Drosophila melanogaster, Mauremys sinensis, Bombyx mori, Ictalurus punctatus, Lotus japonicus, Meretrix petechialis, Ctenopharyngodon, Eospalax cansus, Cotton bollworms, Ctenopharyngodon idellus, broiler embryos, Flounder, Echinococcus multilocularis, Nicotiana benthamiana, Vaccinium dunalianum, Caenorhabditis elegans, Escherichia coli, Hordeum vulgare, Haliotis, turtle, Eleusine indica, Solanum lycopersicum, Ziziphus jujuba, Capra hircus, Brassica napus, juvenile grass, Pimephales promelas, Carassius auratus gibelio, Chlorocebus aethiops, Megalobrama amblycephala, Mus, Mancinella armigera, Anser cygnoides domesticus, Chlorocebus sabaeus, Gallus gallus domesticus, Puerariae Flos, Felis catus, Pampus argenteus, Anser, Ctenopharyngodon idella, Oreochromis mossambicus, Myospalax cansus, Nicotiana tabacum, Fish, Dianthus caryophyllus L., Scylla, Actinopterygii, Mesocricetus auratus, Rattus, Cricetulus migratorius, Cyprinus Carpio, Locusta migratoria, Pelteobagrus fulvidraco, Anser albifrons, Micropterus salmoides, Oreochromis niloticus, Trichopodus trichopterus, Oryctolagus cuniculus, Acanthopagrus schlegelii, Oryza sativa, Mytilus coruscus, Mus musculusRattus norvegicus, Fathead minnow, King Charles Spaniel, Homo sapiensMus musculus, Dianella ensifolia, mus musculus, Cyprinus carpio Linnaeus, Spondyliosoma cantharus, Other, immortalized porcine alveolar macrophage (iPAM) cells, Ovis aries)

IHC (Mus musculus, Homo sapiens, Rattus norvegicus, Oryctolagus cuniculus, Chlorocebus sabaeus)

IP (Mus musculus, Other, Danio rerio, Homo sapiens, Rattus norvegicus)

FC (Sus scrofa)

IF (Sus scrofa, Rattus norvegicus, Mus musculus, Homo sapiens, Danio rerio, Bos taurus)

Co-IP (Alternaria alternata, Mus musculus, Homo sapiens, Zea mays)

qPCR (Gallus gallus domesticus, Mus musculus, Cyprinus carpio, Homo sapiens)

immunoblotting (Mus musculus)

IB (Homo sapiens, Mus musculus)

PE (Mus musculus)

ELISA (Mus musculus)

qRT-PCR (Gallus gallus, Mus musculus)

DB (Mus musculus)

RT-PCR (Homo sapiens)

ChIP (Other)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

β-Actin Rabbit mAb (High Dilution) images

ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)}

Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of various lysates using β-Actin Rabbit mAb (High Dilution) (AC026) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)}

Western blot - β-Actin Rabbit mAb (High Dilution) (AC026)

Western blot analysis of lysates from HeLa cells, using β-Actin Rabbit mAb (High Dilution) (AC026) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on ACTB. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ACTB. (Distance between topics and target gene indicate popularity.) ACTB

* Data provided by citexs.com, for reference only.

Publishing research using AC026? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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