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ACTB Monoclonal Antibody (AC004)

Review (2)Publication (89) Datasheet

ABclonal:Western blot - ACTB Monoclonal Antibody (AC004)
ABclonal:Western blot - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004)
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004)
Review
ABclonal: review for ACTB Monoclonal Antibody(AC004)
Review
ABclonal: review for ACTB Monoclonal Antibody(AC004)

Overview

Product nameACTB Monoclonal Antibody
Catalog No.AC004
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
ImmunogenFusion protein of human β-actin
SequenceEmail for sequence
Gene ID60
Swiss protP60709
SynonymsACTB; BRWS1; PS1TP5BP1; beta actin
Calculated MW41kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:10000 - 1:100000
IHC 1:100- 1:200
IF 1:50- 1:200
Antibody Dilute ProcedurePut 5ul AC004 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, TE-1, 293T, NIH/3T3, Mouse brain, Mouse testis, C6, Rat brain, Rat lung
Cellular locationCytoplasm, cytoskeleton
Customer validation

(, )

WB(Human, unknown, Mouse, Tongue sole, Murine, Pacific oyster, Rat, Human, Citrus sinensis, Pig, grass carp, Rat)

IF(Human, Mouse, Rat)

IP(Human)

IF(Human)

IHC(Human)

Co-IP(Human)

(Human)

ELISA(Mouse)

ABclonal:Western blot - ACTB Monoclonal Antibody (AC004) }

Western blot - ACTB Monoclonal Antibody (AC004)

Western blot analysis of extracts of various cell lines, using β-actin antibody (AC004) at 1:80000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - ACTB Monoclonal Antibody (AC004) }

Western blot - ACTB Monoclonal Antibody (AC004)

Western blot analysis of extracts of NIH/3T3 cells, using β-actin antibody (AC004) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded rat spleen using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded rat heart using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded human stomach using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded mouse liver using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded mouse spleen using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded mouse heart using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded rat liver using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunohistochemistry - ACTB Monoclonal Antibody (AC004) }

Immunohistochemistry - ACTB Monoclonal Antibody (AC004)

Immunohistochemistry of paraffin-embedded rat testis using β-actin antibody (AC004) at dilution of 1:500 (40x lens).
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004) }

Immunofluorescence - ACTB Monoclonal Antibody (AC004)

Immunofluorescence analysis of C6 cells using ACTB Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004) }

Immunofluorescence - ACTB Monoclonal Antibody (AC004)

Immunofluorescence analysis of HeLa cells using ACTB Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ACTB Monoclonal Antibody (AC004) }

Immunofluorescence - ACTB Monoclonal Antibody (AC004)

Immunofluorescence analysis of L929 cells using ACTB Monoclonal Antibody (AC004) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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