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ACE2 Rabbit mAb (A4612)


ABclonal:ACE2 Rabbit mAb
ABclonal:Western blot - ACE2 Rabbit mAb (A4612)
ABclonal:Immunohistochemistry - ACE2 Rabbit mAb (A4612)


Product nameACE2 Rabbit mAb
Catalog No.A4612
Host speciesRabbit
Purification methodAffinity purification
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human ACE2
SequenceEmail for sequence
Gene ID59272
Swiss protQ9BYF1
Calculated MW92kDa
Observed MW100KDa/120KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHepG2, Mouse brain, Mouse heart, Rat brain, Rat kidney
Cellular locationCell membrane, Cytoplasm, Secreted, Single-pass type I membrane protein
ABclonal:Western blot - ACE2 Rabbit mAb (A4612) }

Western blot - ACE2 Rabbit mAb (A4612)

Western blot analysis of extracts of various cell lines, using ACE2 antibody (A4612) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - ACE2 Rabbit mAb (A4612) }

Immunohistochemistry - ACE2 Rabbit mAb (A4612)

Immunohistochemistry of paraffin-embedded mouse kidney using ACE2 Rabbit mAb (A4612) at dilution of 1:100 (40x lens).

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