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ACADS Rabbit pAb (A0945)

Publications (2) Datasheet

ABclonal:Western blot - ACADS Rabbit pAb (A0945)

Western blot analysis of extracts of various cell lines, using ACADS antibody (A0945) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunohistochemistry - ACADS Rabbit pAb (A0945)

Immunohistochemistry of paraffin-embedded human liver cancer using ACADS antibody (A0945) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - ACADS Rabbit pAb (A0945)

Immunohistochemistry of paraffin-embedded human liver damage using ACADS antibody (A0945) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - ACADS Rabbit pAb (A0945)

Immunofluorescence analysis of U2OS cells using ACADS antibody (A0945). Blue: DAPI for nuclear staining.

Overview

Product nameACADS Rabbit pAb
Catalog No.A0945
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ACADS (NP_000008.1).
SequenceMAAALLARASGPARRALCPRAWRQLHTIYQSVELPETHQMLLQTCRDFAEKELFPIAAQVDKEHLFPAAQVKKMGGLGLLAMDVPEELGGAGLDYLAYAIAMEEISRGCASTGVIMSVNNSLYLGPILKFGSKEQKQAWVTPFTSGDKIGCFALSEPGNGSDAGAASTTARAEGDSWVLNGTKAWITNAWEASAAVVFASTDRALQNKGISAFLVPMPTPGLTLGKKEDKLGIRGSSTANLIFEDCRIPKDSILGEPGMG
Gene ID35
Swiss protP16219
SynonymsACADS; ACAD3; SCAD
Calculated MW44kDa
Observed MW44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, HepG2, 293T, HeLa, Mouse liver, Mouse heart, Rat liver
Cellular locationMitochondrion matrix
Customer validation

WB(Sus scrofa, Homo sapiens)

ABclonal:Western blot - ACADS Rabbit pAb (A0945) }

Western blot - ACADS Rabbit pAb (A0945)

Western blot analysis of extracts of various cell lines, using ACADS antibody (A0945) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
ABclonal:Immunohistochemistry - ACADS Rabbit pAb (A0945) }

Immunohistochemistry - ACADS Rabbit pAb (A0945)

Immunohistochemistry of paraffin-embedded human liver cancer using ACADS antibody (A0945) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - ACADS Rabbit pAb (A0945) }

Immunohistochemistry - ACADS Rabbit pAb (A0945)

Immunohistochemistry of paraffin-embedded human liver damage using ACADS antibody (A0945) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - ACADS Rabbit pAb (A0945) }

Immunofluorescence - ACADS Rabbit pAb (A0945)

Immunofluorescence analysis of U2OS cells using ACADS antibody (A0945). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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