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ACADM Rabbit pAb (A1873)

Publications (3) Datasheet

ABclonal:Western blot - ACADM Rabbit pAb (A1873)

Western blot analysis of extracts of various cell lines, using ACADM antibody (A1873) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - ACADM Rabbit pAb (A1873)

Immunofluorescence analysis of HeLa cells using ACADM antibody (A1873) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameACADM Rabbit pAb
Catalog No.A1873
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ACADM (NP_000007.1).
SequenceMAAGFGRCCRVLRSISRFHWRSQHTKANRQREPGLGFSFEFTEQQKEFQATARKFAREEIIPVAAEYDKTGEYPVPLIRRAWELGLMNTHIPENCGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKAPANKAFT
Gene ID34
Swiss protP11310
SynonymsACADM; ACAD1; MCAD; MCADH
Calculated MW46kDa/47kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHT-29, HepG2, U-251MG, K-562, Mouse liver, Mouse kidney, Mouse brain, Mouse heart, Rat liver
Cellular locationMitochondrion matrix
Customer validation

WB(Mus musculus, Sus scrofa)

ABclonal:Western blot - ACADM Rabbit pAb (A1873) }

Western blot - ACADM Rabbit pAb (A1873)

Western blot analysis of extracts of various cell lines, using ACADM antibody (A1873) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - ACADM Rabbit pAb (A1873) }

Immunofluorescence - ACADM Rabbit pAb (A1873)

Immunofluorescence analysis of HeLa cells using ACADM antibody (A1873) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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