Product Type > Antibodies > Secondary Antibodies

ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS039)

Publications (42) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Immunofluorescence - ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS039)

Confocal imaging of HeLa cells using [KD Validated] TIMM10 Rabbit mAb (A24996, dilution 1:200) followed by a further incubation with ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS039, dilution 1:400). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS039)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test, orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L)(AS039, 1:1000) was used as a secondary antibody.

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Overview

Product name ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L)
Catalog No. AS039
Host species Goat
Purification method Affinity purification
Isotype Horseradish peroxidase conjugated IgG

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Immunogen information

Immunogen Rabbit IgG
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 1:100 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.
Application key Immunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF (Rattus norvegicus, Equus asinus, Mus musculus, Homo sapiens, insect, Gallus gallus, Ctenopharyngodon idella, Ctenopharyngodon idellus, rabbit)

IHC (Homo sapiens, Mus musculus)

immunofluorescent (Homo sapiens)

WB (Saccharomyces cerevisiae, Homo sapiens)

Immunofluorescence (Mus musculus)

Immunostaining (Mus musculus)

FC (Oryctolagus cuniculus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) images

ABclonal:Immunofluorescence - ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS039)}

Immunofluorescence - ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS039)

Confocal imaging of HeLa cells using [KD Validated] TIMM10 Rabbit mAb (A24996, dilution 1:200) followed by a further incubation with ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS039, dilution 1:400). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Flow CytoMetry - ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS039)}

Flow CytoMetry - ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L) (AS039)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test, orange line) or secondary antibody only (blue line). Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 594-conjugated Goat Anti-Rabbit IgG (H+L)(AS039, 1:1000) was used as a secondary antibody.

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Related Targets

Based on existing publications, the following genes are closely related to research on Goat Anti-Rabbit. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Goat Anti-Rabbit. (Distance between topics and target gene indicate popularity.) Goat Anti-Rabbit

* Data provided by citexs.com, for reference only.

Publishing research using AS039? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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