Product Type > Antibodies > Secondary Antibodies

ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035)

Publications (9) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Immunofluorescence - ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035)

Immunofluorescence analysis of HeLa cells, using PARP1 antibody (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035) at 1:100 dilution.
Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035)

Flow cytometry:1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test, orange line) or secondary antibody only (blue line).Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L)(AS035, 1:800)was used as a secondary antibody.

You may also interested in:

Overview

Product name ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L)
Catalog No. AS035
Host species Donkey
Purification method Affinity purification
Isotype ABflo™ 488 conjugated IgG

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Immunogen information

Immunogen Rabbit IgG
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • IF/ICC 1:100 - 1:800
  • FC 1:100 - 1:800
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Application key Immunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF (Oryza sativa, Homo sapiens, Rattus norvegicus, M. incognita, Mus musculus)

WB (Mus musculus)

ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) images

ABclonal:Immunofluorescence - ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035)}

Immunofluorescence - ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035)

Immunofluorescence analysis of HeLa cells, using PARP1 antibody (A0942) as the primary antibody at dilution of 1:100. The cells were incubated with the primary antibody overnight at 4°C.
Secondary antibody: ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035) at 1:100 dilution.
Blue: DAPI for nuclear staining.
ABclonal:Flow CytoMetry - ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035)}

Flow CytoMetry - ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L) (AS035)

Flow cytometry:1X10^6 K-562 cells (negative control, left) and A-431 cells (right) were surface-stained with Purified Rabbit anti-Human E-Cadherin mAb(5 μl/Test, orange line) or secondary antibody only (blue line).Non-fluorescently stained K-562 and A-431 cells were used as blank control (red line). ABflo® 488-conjugated Donkey Anti-Rabbit IgG (H+L)(AS035, 1:800)was used as a secondary antibody.

Inquire About This Product

Submit your question about AS035 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on Donkey Anti-Rabbit. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Donkey Anti-Rabbit. (Distance between topics and target gene indicate popularity.) Donkey Anti-Rabbit

* Data provided by citexs.com, for reference only.

Publishing research using AS035? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order