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ABCG1 Rabbit pAb (A4328)

Publications (3) Datasheet

ABclonal:Western blot - ABCG1 Rabbit pAb (A4328)

Western blot analysis of extracts of various cell lines, using ABCG1 Polyclonal Antibody (A4328) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ABCG1 Rabbit pAb (A4328)

Immunohistochemistry of paraffin-embedded human colon carcinoma using ABCG1 Rabbit pAb (A4328) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - ABCG1 Rabbit pAb (A4328)

Immunofluorescence analysis of Rat lung using ABCG1 antibody (A4328) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameABCG1 Rabbit pAb
Catalog No.A4328
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ABCG1 (NP_058198.2).
SequenceMACLMAAFSVGTAMNASSYSAEMTEPKSVCVSVDEVVSSNMEATETDLLNGHLKKVDNNLTEAQRFSSLPRRAAVNIEFRDLSYSVPEGPWWRKKGYKTLLKGISGKFNSGELVAIMGPSGAGKSTLMNILAGYRETGMKGAVLINGLPRDLRCFRKVSCYIMQDDMLLPHLTVQEAMMVSAHLKLQEKDEGRREMVKEILTALGLLSCANTRTGSLSGG
Gene ID9619
Swiss protP45844
SynonymsABC8; WHITE1; ABCG1
Calculated MW71kDa/73kDa/74kDa/75kDa/86kDa
Observed MW100KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2
Cellular locationEndoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - ABCG1 Rabbit pAb (A4328) }

Western blot - ABCG1 Rabbit pAb (A4328)

Western blot analysis of extracts of various cell lines, using ABCG1 Polyclonal Antibody (A4328) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ABCG1 Rabbit pAb (A4328) }

Immunohistochemistry - ABCG1 Rabbit pAb (A4328)

Immunohistochemistry of paraffin-embedded human colon carcinoma using ABCG1 Rabbit pAb (A4328) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - ABCG1 Rabbit pAb (A4328) }

Immunofluorescence - ABCG1 Rabbit pAb (A4328)

Immunofluorescence analysis of Rat lung using ABCG1 antibody (A4328) at dilution of 1:100. Blue: DAPI for nuclear staining.

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