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ABCA1 Rabbit pAb (A7228)

Reviews (2)Publications (5) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - ABCA1 Rabbit pAb (A7228)

Western blot analysis of extracts of HepG2 cells, using ABCA1 antibody (A7228) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - ABCA1 Rabbit pAb (A7228)

Western blot analysis of extracts of various cell lines, using ABCA1 antibody (A7228) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - ABCA1 Rabbit pAb (A7228)

Immunofluorescence analysis of C6 cells using ABCA1 Rabbit pAb (A7228) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ABCA1 Rabbit pAb (A7228)

Immunofluorescence analysis of NIH/3T3 cells using ABCA1 Rabbit pAb (A7228) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for ABCA1 Rabbit pAb(A7228)
Review
ABclonal: review for ABCA1 Rabbit pAb(A7228)

Overview

Product nameABCA1 Rabbit pAb
Catalog No.A7228
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 240-470 of human ABCA1 (NP_005493.2).
SequenceLRTLNSTSPFPSKELAEATKTLLHSLGTLAQELFSMRSWSDMRQEVMFLTNVNSSSSSTQIYQAVSRIVCGHPEGGGLKIKSLNWYEDNNYKALFGGNGTEEDAETFYDNSTTPYCNDLMKNLESSPLSRIIWKALKPLLVGKILYTPDTPATRQVMAEVNKTFQELAVFHDLEGMWEELSPKIWTFMENSQEMDLVRMLLDSRDNDHFWEQQLDGLDWTAQDIVAFLAKH
Gene ID19
Swiss protO95477
SynonymsABCA1; ABC-1; ABC1; CERP; HDLDT1; TGD
Calculated MW254kDa
Observed MW254KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHepG2, U-87MG, Mouse liver, Mouse ovary, Mouse uterus, Rat liver, Rat testis, Rat uterus
Cellular locationMembrane, Multi-pass membrane protein
Customer validation

(Human hepatoma cells)

WB(Mus musculus, Homo sapiens)

IF(Mus musculus)

IF(Homo sapiens)

IHC(Homo sapiens)

ABclonal:Western blot - ABCA1 Rabbit pAb (A7228) }

Western blot - ABCA1 Rabbit pAb (A7228)

Western blot analysis of extracts of HepG2 cells, using ABCA1 antibody (A7228) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - ABCA1 Rabbit pAb (A7228) }

Western blot - ABCA1 Rabbit pAb (A7228)

Western blot analysis of extracts of various cell lines, using ABCA1 antibody (A7228) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - ABCA1 Rabbit pAb (A7228) }

Immunofluorescence - ABCA1 Rabbit pAb (A7228)

Immunofluorescence analysis of C6 cells using ABCA1 Rabbit pAb (A7228) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ABCA1 Rabbit pAb (A7228) }

Immunofluorescence - ABCA1 Rabbit pAb (A7228)

Immunofluorescence analysis of NIH/3T3 cells using ABCA1 Rabbit pAb (A7228) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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