Baculovirus Expression System

  • The baculovirus expression vector system (BEVS) is a powerful system for the production of high quality proteins. Although time lines are increased compared to E. coli, proteins produced in BEVS tend to be soluble and functional and carry post-translational modifications like phosphorylation and glycosylation. The system gives very high recombinant protein yields and we often recommend it for proteins that are difficult to express in E. coli.
  • ABclonal has a rich experience in choosing the ways of intracellular or secretory expression , signal peptide, the corresponding kind of insect cell line such as Sf9, Sf21, Hi-5 and the culture method,according to the reformed classic Bac-to-Bac expression system.

Insect Expression Flowchart

Highlights


  • High success rate

    ABclonal has not yet failed cases with the Baculovirus-insect Expression System.

  • Advanced optimization system

    Free and advanced codon optimization specific to Sf9, Sf21, Hi-5 insect cell lines are provided by ABclonal based on the nature of the protein. We can choose the best possible protocol to get the highest expression amount by detecting multiplicity of infection at different time points.

  • Large-scale production

    ABclonal can scale up 200 L to express and purify protein.

  • One-stop service

    From gene to protein.Design and optimize gene, virus production, express and optimize protein, protein purification and post processing, management of data and report.

Package Details


Service Name Service Content Timeline
Plasmid Construction
  • Gene synthesis with codon optimization& subclone
  • Identification of recombinant Bacmid DNA

 

3-5 Weeks
Virus Proliferation
  • Transfection of insect cell with recombinant Bacmid DNA
  • Generation of P1 stock (low titer), P2 stock (high titer), P3 stock (higher titer), and the virus titer detectived by quantitative-PCR

 

2-3 Weeks
Protein Expression Test
  • Infection of insect cells with P2 or P3 stock
  • Protein affinity purification(or other purification method)
  • QC the purified protein

 

2-3 Weeks
Large-scale expression & purification
  • Infection of insect cells with P2 or P3 stock
  • Protein affinity purification(or other purification method)
  • Tag free protein (if required)
  • QC the purified protein
1-10L 2-3 Weeks
10-20L 3 Weeks
20-50L 3-4 Weeks
Above 50L 6-14 Weeks

Note:

QC: SDS-PAGE/WB identification of the purified protein,MS identification(if required).

All service timelines are approximate. In most cases, we finish the custom project within the appointed time. However, optional steps might prolong the deliver timeline.

Custom protein service is charged step by step and save your valuable research costs.

Protein Service Quotation Request Form
  • For quotation requests and questions, you may contact us by email, phone, or via our online quotation system.
  • Orders can be placed by phone, email, fax, or online with a formal Purchase Order or credit card.
  • Please send your samples to NeoScientific, 86 Cummings Park, Woburn, Massachusetts.
  • Our customer service is available 24hours per day/7 days a week/365 days a year.

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