Bacterial Expression service
The advantages of E. coli expression systems include low cost, high expression, ease of scale up, and short turnaround time. Our collection of more than 10 host strains and numerous expression vectors guarantees selection of the most efficient expression system. To increase protein solubility, expression levels, and ease of purification, multiple fusion/affinity tags are available. Our proprietary AIE technology allows high yields of toxic proteins. For insoluble proteins we have developed an effective protein refolding system consisting of 20 refolding buffers optimized with 9 parameters. If large amounts of protein are required, we can produce and purify from cultures as large as 35-500 liters. Metabolic labeling with stable isotopes is also offered in the E. coli system.
E.coli Expression Flowchart
High success rate
The success rate of the soluble protein expression provided by ABclonal using E. coli can be up to 95%.
Optional expression vectors
A various of screened or reformed
E. coli expression vectors are provided by ABclonal, including pET series, pBAD series ,pGEX series and pCold series, to meet the specific requirements of intracellular and periplasmic space secretory expression.
Optional protein tags
ABclonal use solubilized tags such as SUMO, Trx, GST and NusA to improve hydrophilicity of protein in inclusion body expression.
Optional expression hosts
More than 10 E. coli expression hosts, including standard expression strain, protein expression enhanced strain, toxic protein expression strain, ultra-low temperature strain for enhancing soluble proteins expression and other expression strains reformed by ABclonal can be used to meet customers’ different requirement.
1-4000L per batch.
From gene to protein. Design and optimize gene, optimize protein expression conditions, protein purification and post processing, management of data and report.
| Plasmid Construction
- Gene synthesis with codon optimization& subclone
| Protein Expression Test
- Plasmid transformation
- Exploration of expression condition,expression analysis and identification(SDS-PAGE and /or WB)
- 200ml amplification in optimum condition and purification test
| Large-scale fermentation & purification
- Scale up protein expression using optimized conditions
- Protein affinity purification (desired protein amounts and purity)
- Inclusion bodies denaturation & renaturation(if required)
- Tag-free protein (if required), endotoxin removing(if required)
- QC the purified protein
QC: The purified protein is identified by SDS-PAGE/WB and MS (if required).
All service timelines are approximate. In most cases, we finish the custom project within the appointed time. However, optional steps might prolong the deliver timeline.
Custom protein service is charged step by step and save your valuable research costs.